Ultrastructural Visualization of the Nuclear Envelope in HeLa Cells.

Sourabh Sengupta, Zhaojie Zhang, Daniel L Levy

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sourabh SenguptaDepartment of Molecular Biology, University of Wyoming, Laramie, WY, USA.

Zhaojie ZhangDepartment of Zoology and Physiology, University of Wyoming, Laramie, WY, USA.

Daniel L LevyDepartment of Molecular Biology, University of Wyoming, Laramie, WY, USA. dlevy1@uwyo.edu.

Funding

Wyoming INBRE Phase 4- Equipment Supplement for x-ray diffractometer for Center for Advanced Scientific InstrumentationP20GM103432 · NIGMS · UNIVERSITY OF WYOMING · PI Nicolas A. Blouin · 2012 to 2026

$56.8M

Mechanisms of nuclear size regulationR35GM134885 · NIGMS · UNIVERSITY OF WYOMING · PI Daniel Leon Levy · 2020 to 2026

$2.5M

NIGMS NIH HHS P20 GM103432NIGMS NIH HHS R35 GM134885

6 · The paper itself

Abstract

Transmission electron microscopy (TEM) is a powerful tool that has been used for the visualization of biological materials since the 1940s. This technique permits the detailed observation of cellular organelles and other intracellular structures as well as infectious agents, like viruses. Important insights about the structure and function of the cell nucleus have been gleaned using TEM. Here we present a method for visualization of the nuclear envelope in cultured HeLa cells using TEM. The method covers cell culturing, fixation, embedding, sectioning, staining, and visualization. This approach is particularly useful to understand how the ultrastructure of the nuclear envelope is affected by cell culture conditions, genetic manipulations, and stress treatments.

Indexed as

Microscopy, Electron, TransmissionNuclear EnvelopeHeLa CellsHumansStaining and LabelingHeLa cellsNuclear envelopeNucleusTransmission electron microscopy

Identifiers

PMID40833565

DOI10.1038/s41592-019-0375-1

PMCPMC12431628

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.