ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025
A Genetically Encoded Biosensor for Characterizing Transport and Metabolism of Glutarate.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Advances and perspectives in genetically encoded biosensors for biomarker detection and theranostic circuits.Synthetic and systems biotechnology · 2027Review
- Affinity Peptide-Based Circularly Permuted Fluorescent Protein Biosensors Loaded in a Microfluidic System for Systemic Lupus Erythematosus Diagnosis.Sensors (Basel, Switzerland) · 2026Article
- A Genetically Encoded Biosensor for Characterizing Transport and Metabolism of Glutarate.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
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Authors and funding
13 authors.
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Abstract
Glutarate is a platform chemical with diversified applications. It is also an endogenous metabolite involved in various physiological processes. Deficiency in glutaryl-CoA dehydrogenase (GcdH) for glutarate catabolism induces the inherited metabolic disorder glutaric aciduria. In this study, a genetically encoded glutarate fluorescent biosensor Glusor is constructed and optimized based on transcriptional regulator CsiR and circularly permuted yellow fluorescent protein. Glusor can quantify glutarate in human body fluids and bacteria fermentation broth with good accuracy and precision, supporting the convenient diagnosis of glutaric aciduria and glutarate production monitoring. Then, the glutarate transport is characterized independent of radioactive substrate by using Glusor expressed in Escherichia coli. The functions of transporters KgtP and YnfM in the uptake and efflux of glutarate in E. coli are identified. Glusor is also used to reveal the catabolism of glutarate in bacteria and HEK293FT cells. The role of glutarate hydroxylase in glutarate catabolism of E. coli is identified through Glusor-supported in situ glutarate detection. Spatially resolved in vivo analysis of glutarate in HEK293FT cells is realized and GcdH inhibition and hypoxia-induced glutarate accumulation are elucidated by using Glusor. Overall, Glusor is a versatile tool for the detection of glutarate both in vitro and in vivo.
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Registered trials
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