The improvement effect of insoluble dietary fiber of

Objective: Methods: Male C57BL/6 J mice were fed with a high-fat diet continuously for 8 weeks. At the same time, the low-dose and high-dose groups were supplemented with 0.5 g/kg·BW and 1.0 g/kg·BW of PIDF, respectively. The weight and food intake of the mice were measured during the experiment. After 8 weeks of feeding, the organ weight, serum indexes, and liver function were investigated. Furthermore, the mechanism of antihyperlipidemic was explained by analyzing the gut microbiota and metabolites. Results: The results of the LIDF and HIDF showed that the PIDF treatment significantly alleviated the liver and kidney weight and body fat index. PIDF administration remarkably increased the high-density lipoprotein cholesterol level and enhanced hepatic superoxide dismutase activity in high-fat diet-fed mice. The levels of total cholesterol, triglycerides, low-density lipoprotein cholesterol, glucose, and aspartate transaminase in the HIDF were significantly lower than in the high-fat diet group. In addition, PIDF supplements also decreased the ratio of Conclusion: It can be concluded that PIDF may alleviate hyperlipidemia by regulating cholesterol metabolism, increasing the abundance of beneficial microorganisms, and controlling its metabolites. The results of this study accelerated the application of PIDF in the health food industry.