Evidence map›Paper›PMID 40832265›Full record

ArticlebioRxiv : the preprint server for biology2025

Exploring the deletion landscape of

Andrew J Plebanek, Luke M Oltrogge, Cynthia I Terrace, Maria Lukarska, Maggie Khoury, David F Savage

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Andrew J PlebanekDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Luke M OltroggeDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Cynthia I TerraceDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Maria LukarskaDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Maggie KhouryDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
David F SavageDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.ORCID 0000-0003-0042-2257

Funding

Engineering CRISPR-Cas proteins for conditional and robust interrogation of the genomeR01GM127463 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI SAVAGE, DAVID FRANK · 2019 to 2022
$1.2M
NIGMS NIH HHS R01 GM127463
6 · The paper itself

Abstract

Profiling tolerated amino acid deletions in proteins can elucidate structure-function relationships, reconstruct intermediate stages in protein evolution, and be used to engineer minimized versions of proteins with size-sensitive biotechnology applications. Despite advances in deletion library construction techniques over the past several decades, there are presently few methods available that are simultaneously efficient, precise, and easy to implement. Here we present SABER, a novel approach which utilizes SpRYCas9, a near-PAMless engineered SpCas9 variant, as a molecular biological tool for building deletion libraries with unprecedented speed and ease. We applied this technique to the small and structurally divergent Cas9 from

Identifiers

PMID40832265
PMCPMC12363808

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.