Evidence map›Paper›PMID 40832163›Full record

ArticlebioRxiv : the preprint server for biology2025

LENG8 mediates RNA nuclear retention and degradation in eukaryotes.

Lusong Tian, Liang Liu, Yoseop Yoon, Lindsey V Soles, Marielle Valdez, Joshua Jeong, Clinton Yu, Lan Huang, Yongsheng Shi

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Lusong TianDepartment of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92617, USA.
Liang LiuDepartment of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92617, USA.
Yoseop YoonDepartment of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92617, USA.
Lindsey V SolesDepartment of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92617, USA.
Marielle ValdezDepartment of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92617, USA.
Joshua JeongDepartment of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92617, USA.
Clinton YuDepartment of Physiology and Biophysics, University of California, Irvine, Irvine, CA 92697, USA.
Lan HuangDepartment of Physiology and Biophysics, University of California, Irvine, Irvine, CA 92697, USA.
Yongsheng ShiDepartment of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92617, USA.

Funding

Univ.of Calif., Irvine Cancer Center Support GrantP30CA062203 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Melanie Funes · 1994 to 2026
$57.9M
Supplement: Advancing Proteomics Technologies to Decipher the Ubiquitin-Proteasome SystemR35GM145249 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI Lan Huang · 2022 to 2026
$3.2M
Mechanisms and regulation of mRNA 3' processingR35GM149294 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI Yongsheng Shi · 2023 to 2026
$2.1M
NCI NIH HHS P30 CA062203NIGMS NIH HHS R35 GM145249NIGMS NIH HHS R35 GM149294
6 · The paper itself

Abstract

In eukaryotes, incompletely processed and misprocessed mRNAs as well as numerous noncoding RNAs are retained in the nucleus and often degraded. However, the mechanisms for this critical quality control pathway remain poorly understood. Here we identify LENG8 as a conserved RNA nuclear retention factor. We showed that LENG8 is recruited to pre-mRNAs by splicing factors, including the U1 snRNP. LENG8 binds to PCID2 and SEM1 to form the REX (Repressor of EXport) complex, which is conserved from yeast to human, and causes RNA nuclear retention by acting as a dominant negative factor for the essential mRNA export factor TREX-2. LENG8 depletion leads to the leakage of misprocessed mRNAs, including intronically polyadenylated and intronretained mRNAs, as well as noncoding RNAs into the cytoplasm. Finally, LENG8 promotes RNA degradation by recruiting PAXT and the RNA exosome. Thus our study revealed a conserved quality control mechanism for eukaryotic gene expression that ensures only fully and correctly processed RNAs are exported from the nucleus.

Indexed as

intron retentionRNA degradationRNA exportRNA nuclear retentionRNA polyadenylationsplicing

Identifiers

PMID40832163
PMCPMC12363940

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.