Evidence map›Paper›PMID 40831826›Full record

ArticleCureus2025

Estimation of Alkaline Phosphatase Activity in Ovarian Stem Cells With and Without the Use of Additives Replacing Fetal Bovine Serum.

Sindhuja Srinivasan, Sanjeeva Reddy, Alan Mathew Punnoose, Madan Kalagara, Mubeena S, Paramesh Vishwanathan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cureus, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sindhuja SrinivasanReproductive Medicine and Surgery, Sri Ramachandra Institute of Higher Education and Research, chennai, IND.
Sanjeeva ReddyReproductive Medicine and Surgery, Sri Ramachandra Institute of Higher Education and Research, Chennai, IND.
Alan Mathew PunnooseStem Cell and Regenerative Biology Laboratory, Sri Ramachandra Institute of Higher Education and Research, Chennai, IND.
Madan KalagaraPathology, Vijaya Medical Centre, Vishakapatnam, IND.
Mubeena SStem Cell and Regenerative Biology Laboratory, Sri Ramachandra Institute of Higher Education and Research, Chennai, IND.
Paramesh VishwanathanStem Cell and Regenerative Biology Laboratory, Sri Ramachandra Institute of Higher Education and Research, Chennai, IND.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ovarian stem cells (OSCs) are a promising tool for reproductive regeneration, but ethical concerns surrounding fetal bovine serum (FBS) use have prompted the search for viable alternatives. This study estimates alkaline phosphatase (ALP) activity-a pluripotency marker-in two OSC lines (OTC-5, OTC-7) cultured with FBS and with potential replacements: cord blood serum (CB), follicular fluid (FF), and pooled platelet lysate (PP). ALP activity was quantified using a colorimetric p-nitrophenyl phosphate assay. Results show that FF and PP can maintain or slightly improve ALP activity compared to controls, supporting their feasibility as xeno-free culture additives for OSCs.

Indexed as

alkaline phosphatasefetal bovine serumfollicular fluidovarian stem cellspluripotencypooled platelet lysateumbilical cord blood

Identifiers

PMID40831826
PMCPMC12359014

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.