Evidence map›Paper›PMID 40831258›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

A Study on the Cell Layer Patterns of a Citrus Periclinal Chimera Reveals β-Cryptoxanthin Regulation in Citrus Fruits.

Chi Zhang, Kaijie Zhu, Zhehui Zhang, Huiyu Ji, Qun Wu, Lin Zhang, Fuzhi Ke, Gang Wang, Min Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Chi ZhangNational Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, Zhejiang, 311300, P. R. China.ORCID https://orcid.org/0000-0003-3760-7917
Kaijie ZhuNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.ORCID https://orcid.org/0000-0002-9508-175X
Zhehui ZhangNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Huiyu JiNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Qun WuQuzhou Academy of Agricultural and Forestry Sciences, Quzhou, Zhejiang, 324000, P. R. China.
Lin ZhangZhejiang Agricultural Technology Extension Center, Hangzhou, Zhejiang, 310020, P. R. China.
Fuzhi KeZhejiang Citrus Research Institute, Taizhou, Zhejiang, 318026, P. R. China.
Gang WangAgriculture and Rural Bureau of Changshan County, Quzhou, Zhejiang, 324200, P. R. China.
Min ZhangNational Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, Zhejiang, 311300, P. R. China.ORCID https://orcid.org/0000-0003-0043-3810

Funding

Central Agricultural Management Main Capacity Improvement Fund Project 2024ZDXT04Kecheng District Citrus Industry Science and Technology Research Project [2022] 22-3Kecheng District Science and Technology Project ZJZX2023021Public Projects of Zhejiang province LGN22C150006Zhejiang Provincial Science and Technology Cooperation Project 2023SNJF014Zhejiang Provincial Science and Technology Cooperation Project 2023SNJF020
6 · The paper itself

Abstract

As a dietary provitamin A carotenoid, β-cryptoxanthin is more bioavailable than common carotenoids. However, β-cryptoxanthin is a minor carotenoid in most crops, except for certain citrus species. A chimera (OCC) is identified, which grows from the graft junction between mandarin (OOO) and grapefruit (CCC), and it exhibits a unique β-cryptoxanthin profile. OCC is a periclinal chimera with the L1 cell layer from OOO and L2 and L3 layers from CCC. The flavedo, pulp, and segment membranes of OCC are generated from all three cell layers, but the proportions of these cell layers differ; OOO:CCC (L1:L2/L3) is ≈1:4 in the flavedo, 1:1 in the pulp, and 1:3 in the segment membrane. A nucleus-localized transcriptional activator, MYB107, is identified, which regulates β-cryptoxanthin variation between OCC and its donors. MYB107 expression is closely associated with β-cryptoxanthin accumulation, and its overexpression in citrus calli and fruits enhance carotenoid biosynthesis and upregulate carotenogenic genes, whereas MYB107 expression interference demonstrates the opposite effects. MYB107 directly binds to and activates the promoters of β-carotene hydroxylase (BCH), positively regulating β-cryptoxanthin biosynthesis. These findings provide new insights into β-cryptoxanthin accumulation patterns in citrus, highlighting the potential to improve the nutritional and aesthetic value of citrus fruits.

Indexed as

Beta-CryptoxanthinCitrusFruitCarotenoidsChimeraGene Expression Regulation, PlantPlant ProteinsBeta-CryptoxanthinCarotenoidsPlant Proteinscarotenoidchimeracitrusfruit developmentgraftβ‐cryptoxanthin

Identifiers

PMID40831258
PMCPMC12591197

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.