Evidence map›Paper›PMID 40830847›Full record

ArticleBMC infectious diseases2025

Bacterial isolates and antibiotic resistance in critically ill sepsis patients at a tertiary hospital in Ethiopia.

Girum Tesfaye Kiya, Zeleke Mekonnen, Elsah Tegene Asefa, Esayas Kebede Gudina, Hanan Ahmed, Getenet Beyene, Mekidim Mekonnen, Gemeda Abebe

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Article in BMC infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

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0cells of the map it votes in
3citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Girum Tesfaye KiyaSchool of Medical Laboratory Science, Institute of Health, Jimma University, Jimma, Ethiopia. tesfaye.girum@ju.edu.et.
Zeleke MekonnenSchool of Medical Laboratory Science, Institute of Health, Jimma University, Jimma, Ethiopia.
Elsah Tegene AsefaDepartment of Internal Medicine, Institute of Health, Jimma University, Jimma, Ethiopia.
Esayas Kebede GudinaDepartment of Internal Medicine, Institute of Health, Jimma University, Jimma, Ethiopia.
Hanan AhmedMicrobiology Unit, Jimma University Medical Center, Jimma University, Jimma, Ethiopia.
Getenet BeyeneSchool of Medical Laboratory Science, Institute of Health, Jimma University, Jimma, Ethiopia.
Mekidim MekonnenSchool of Medical Laboratory Science, Institute of Health, Jimma University, Jimma, Ethiopia.
Gemeda AbebeSchool of Medical Laboratory Science, Institute of Health, Jimma University, Jimma, Ethiopia.

Funding

Research and Innovation Director (RID) office of Institute of Health, Jimma University Mega Research Fund Scheme (2022-2025)
6 · The paper itself

Abstract

backgroundSepsis is a pressing global public health issue, particularly among patients admitted to intensive care unit (ICU), and it is linked to a high incidence of morbidity and mortality. Current understanding of bacterial profiles, antibiotic resistance patterns, and the magnitude of Extended-Spectrum Beta-Lactamase (ESBL) and Carbapenemase (CP) producing isolates in critically ill patients is vital for implementing strong management approaches tailored to combat antibiotic resistance and improve patient outcomes.

methodsA prospective cross-sectional study was carried out involving 149 patients admitted to ICU with a presumptive diagnosis of sepsis at Jimma University Medical Center (JUMC) from October 2023 to October 2024. A structured questionnaire was administered to collect baseline demographic and clinical characteristics of the patients. Blood samples were aseptically collected and analyzed using standard microbiological technique to identify bacterial isolates. The Kirby-Bauer disc diffusion method was employed to conduct the antimicrobial susceptibility test. The MASTDISCS

resultsBacterial pathogens were isolated from 33 patients, representing an isolation rate of 22.1% (95% CI: 16.2-29.6%). Gram-negative bacteria (GNB) accounted for the majority of the isolates, 25/33 (75.8%), with Escherichia coli, 6/25 (24%) being the predominant species. Administering antibiotics before sample collection was associated with a lower likelihood of culture positivity (AOR: 0.05, 95% CI: 0.01-0.38; p = 0.004). In contrast, prolonged stay in the ICU was positively associated with culture positivity (AOR: 1.13, 95% CI:1.02-1.24; p = 0.02). Gram-negative bacteria exhibited a particularly high resistance to third- and fourth-generation cephalosporins, ranging from 66.7 to 80%. ESBL and CP producers accounted for 11/25 (44%) and 12/25(48%) of GNB, respectively. Additionally, 80% of Staphylococcus aureus isolates were methicillin resistant. Overall, about a third of all isolates, 11/33 (33.3%) were multidrug resistant strains.

conclusionsThe study shows a significant burden of MDR pathogens among critically ill sepsis patients, characterized by high prevalence of ESBL, CP, and MRSA. These findings underscore the urgent need to strengthen antibiotic stewardship and infection prevention strategies to curb resistance in ICU environments. CLINICAL TRIAL NUMBER: Not applicable.

Indexed as

Anti-Bacterial AgentsBacteriaDrug Resistance, BacterialSepsisAdultAgedbeta-LactamasesCritical IllnessCross-Sectional StudiesEthiopiaFemaleHumansIntensive Care UnitsMaleMicrobial Sensitivity TestsMiddle AgedAnti-Bacterial Agentsbeta-LactamasesAntimicrobial resistanceBacterial isolatesCritically illEthiopiaSepsis

Identifiers

PMID40830847
PMCPMC12366403

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