Evidence map›Paper›PMID 40830242›Full record

Observational studyLung2025

Sputum Transcriptomic Analysis and Clustering Reveals Insight Into Asthma Heterogeneity.

Janne Goossens, Anne-Charlotte Jonckheere, Sien De Boodt, Ellen Dilissen, Nora Marain, Tatjana Decaesteker, Alvaro Cortes, Jeroen A Vanoirbeek, Sven F Seys, Lieven Dupont and 1 more

Registry-linked trialAbstract readObservational Study
PubMed Publisher
In one paragraph

Observational study in Lung, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01224938 (Development and Validation of a Sputum Biomarker mRNA Panel for the Diagnostic Work-up of Asthma 1.), which is not on this map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT01224938 completednot on this map

Development and Validation of a Sputum Biomarker mRNA Panel for the Diagnostic Work-up of Asthma 1.

TypeobservationalSponsorUniversitaire Ziekenhuizen KU LeuvenRan2010 to 2013Enrolled395ConditionsAsthma Patients, Healthy Subjects
3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Janne Goossens *KU Leuven, Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immunology Research Group, Herestraat 49 Box 811, 3000, Leuven, Belgium.ORCID http://orcid.org/0000-0002-9383-8886
Anne-Charlotte Jonckheere *KU Leuven, Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immunology Research Group, Herestraat 49 Box 811, 3000, Leuven, Belgium.ORCID http://orcid.org/0000-0003-4943-892X
Sien De BoodtKU Leuven, Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immunology Research Group, Herestraat 49 Box 811, 3000, Leuven, Belgium.
Ellen DilissenKU Leuven, Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immunology Research Group, Herestraat 49 Box 811, 3000, Leuven, Belgium.ORCID http://orcid.org/0000-0001-9837-8148
Nora MarainKU Leuven, Department of Chronic Diseases and Metabolism, Laboratory of Respiratory Diseases and Thoracic Surgery, Leuven, Belgium.ORCID http://orcid.org/0000-0003-0589-5205
Tatjana DecaestekerKU Leuven, Department of Chronic Diseases and Metabolism, Laboratory of Respiratory Diseases and Thoracic Surgery, Leuven, Belgium.ORCID http://orcid.org/0000-0002-3268-9397
Alvaro CortesKU Leuven, Department of Human Genetics, Laboratory for Cytogenetics and Genome Research, Leuven, Belgium.
Jeroen A VanoirbeekKU Leuven, Department of Chronic Diseases and Metabolism, Laboratory of Respiratory Diseases and Thoracic Surgery, Leuven, Belgium.ORCID http://orcid.org/0000-0001-5508-3518
Sven F SeysKU Leuven, Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immunology Research Group, Herestraat 49 Box 811, 3000, Leuven, Belgium.ORCID http://orcid.org/0000-0002-4399-9892
Lieven DupontKU Leuven, Department of Chronic Diseases and Metabolism, Laboratory of Respiratory Diseases and Thoracic Surgery, Leuven, Belgium.ORCID http://orcid.org/0000-0003-3961-1522
Dominique M A BullensKU Leuven, Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immunology Research Group, Herestraat 49 Box 811, 3000, Leuven, Belgium. dominique.bullens@kuleuven.be.ORCID http://orcid.org/0000-0002-4595-9579

Funding

Fonds Wetenschappelijk Onderzoek 1S20418NOnderzoeksraad, KU Leuven C24/18/092
6 · The paper itself

Abstract

introductionAsthma is a heterogenous disease shaped by different inflammatory pathways. The aim is to investigate transcriptomic profiles in asthmatic patients and associate these with inflammation, airway damage and lung function.

methodsAdult asthma patients attending the outpatient pneumology clinic in our tertiary center, underwent diagnostic sputum induction and upon consent remaining sputum RNA was used for bulk RNA-sequencing (n = 56) coupled with unsupervised clustering. A retrospective analysis of comorbidities was performed. Sputum cytokine mRNA levels were determined via qPCR. Airway damage markers were determined in sputum supernatant

resultsUnsupervised clustering subdivided all asthmatic patients in one of three clusters. Cluster 1 contained most of the pauci-granulocytic asthma patients in whom oxidative stress was upregulated and TLR-signalling and several cytokine pathways down-regulated. Cluster 2 had upregulated S100 family signalling pathway genes, was mostly associated to type 2 inflammation with elevated sputum eosinophils, epithelial damage, IL-4 mRNA levels and allergy. Asthma patients in cluster 3 had worse lung function, upregulated inflammatory genes, increased sputum neutrophils and calprotectin levels.

conclusionThree different asthma clusters could be identified bridging over the classical type 2/non-type 2 classification. CLINICAL TRIAL: ClinicalTrials.gov Identifier: NCT01224938 registered on 19 October 2010.

Indexed as

AsthmaSputumTranscriptomeAdultAgedCluster AnalysisCytokinesFemaleGene Expression ProfilingHumansMaleMiddle AgedRetrospective StudiesRNA, MessengerCytokinesRNA, MessengerAirway inflammationAsthmaComorbiditiesSputum transcriptomics

Identifiers

PMID40830242

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.