Evidence map›Paper›PMID 40828036›Full record

ArticleEuropean journal of immunology2025

TLR9-Driven S-Palmitoylation in Dendritic Cells Reveals Immune and Metabolic Protein Targets.

Juan N Quiroz, Malte Sielaff, Daria Kondrateva, Fatima Boukhallouk, Gloria J Godoy, Cecilia R Molina, Brecht Moonen, Claudia C Motran, Jeroen Bogie, Hugo D Luján and 3 more

Abstract read
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In one paragraph

Article in European journal of immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Juan N QuirozInstitute of Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
Malte SielaffInstitute of Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
Daria KondratevaInstitute of Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
Fatima BoukhalloukInstitute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
Gloria J GodoyInstitute of Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
Cecilia R MolinaInstitute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
Brecht MoonenHasselt University, Department of Immunology and Infection, Biomedical Research Institute, Diepenbeek, Belgium.
Claudia C MotranDepartamento De Bioquímica Clínica, Facultad De Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
Jeroen BogieHasselt University, Department of Immunology and Infection, Biomedical Research Institute, Diepenbeek, Belgium.
Hugo D LujánInstitute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
Stefan TenzerInstitute of Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
Tim SparwasserResearch Center for Immunotherapy (FZI), University Medical Center Mainz, Mainz, Germany.
Luciana BerodInstitute of Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.

Funding

Boehringer Ingelheim Fonds Travel GrantDeutsche Forschungsgemeinschaft CRC156Deutsche Forschungsgemeinschaft Project-ID-318346496-SFB1292(TP18-Q01)Deutsche Forschungsgemeinschaft Project-ID-490846870-TRR355(IRTG-TPB08-TPA04)
6 · The paper itself

Abstract

Dendritic cells (DCs) rely on Toll-like receptor 9 (TLR9) to detect unmethylated CpG motifs in microbial DNA, triggering essential immune responses. While the downstream signaling pathways of TLR9 activation are well characterized, their impact on S-palmitoylation is unknown. S-palmitoylation, involving the reversible attachment of palmitic acid to cysteine residues, plays a crucial role in regulating protein function and is catalyzed by the ZDHHC family of palmitoyl-acyltransferases (PATs). In this study, we investigated the S-palmitoylated proteome of bone marrow-derived GM-CSF DCs (GM-DCs) at resting and following TLR9 activation with CpGB. Using the click-chemistry-compatible analog 17-octadecynoic acid (17-ODYA) and mass spectrometry (MS)-based proteomics, we characterized dynamic remodeling of S-palmitoylation in response to TLR9 activation. This included enrichment of targets involved in immune and metabolic pathways. Transcriptomic analysis of mice and human DCs revealed TLR9-driven modulation of PAT-encoding genes. Subsequently, we explored the contribution of Zdhhc9 expression to the regulation of S-palmitoylation in DCs. Using gene knockout approaches, we identified candidate protein targets potentially linked to ZDHHC9 activity. Interestingly, modulation of Zdhhc9 expression alone did not influence DC maturation, suggesting that other PATs might compensate for its activity. Together, our findings reveal a novel layer of regulation in TLR9 signaling mediated by S-palmitoylation.

Indexed as

AcyltransferasesDendritic CellsLipoylationToll-Like Receptor 9AnimalsHumansMiceMice, Inbred C57BLMice, KnockoutProteomeProteomicsSignal TransductionAcyltransferasesProteomeTLR9 protein, humanTlr9 protein, mouseToll-Like Receptor 9dendritic cellsinnate immunityS‐palmitoylationTLR9 signaling

Identifiers

PMID40828036
PMCPMC12363430

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.