ArticleScience progress
Investigating miRNA-driven DNA methylation: Statistical evidence of gene-specific modulation.
Article in Science progress. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
ObjectiveDNA methylation is a key regulator of gene expression and plays a crucial role in cancer development. However, the mechanisms driving gene-specific methylation remain unclear. This study investigates the role of microRNAs (miRNAs) in regulating promoter methylation of specific genes, aiming to uncover miRNA-driven modulation of gene methylation in cancer.MethodsWe analyzed data from the Cancer Cell Line Encyclopedia (CCLE) database, comprising 813 cell lines. Spearman's rank correlation was performed between the expression levels of 734 miRNAs and the methylation levels of 20,587 genes, focusing on CpG islands in promoter regions. Linear regression analysis was used to validate the relationship between selected miRNAs and gene clusters. Bioinformatics screening identified statistically significant miRNA-gene pairs involved in promoter methylation.ResultsThe analysis revealed 25 target genes whose promoter methylation was significantly associated with the expression of four miRNAs (hsa-miR-200a, hsa-miR-200b, hsa-miR-200c, and hsa-miR-141). These correlations were most pronounced in colorectal, gastric, lung, and ovarian cancers. Notably, cancer-related genes such as ST14, OVOL1, and EPCAM were identified as targets, supporting the hypothesis that miRNAs regulate promoter methylation in these genes.ConclusionOur findings suggest that specific miRNAs induce promoter methylation in cancer-related genes, thereby influencing gene expression. This study expands our understanding of the role of miRNAs in tumor development and highlights the potential of miRNA-based therapies in cancer treatment. As this is a computational study, further experimental validation is required to confirm the proposed regulatory mechanisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.