Evidence map›Paper›PMID 40826158›Full record

ArticleScientific reports2025

Efficient extracellular expression and rapid screening of human-like recombinant gelatin in Komagataella phaffii.

Xiaoping Song, Yajie Wang, Kaiwei Kan, Zhenbo Chai, Liping Wang, Mingyang Li, Zhangqi Liu

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Xiaoping SongDepartment of Pharmacy, Anhui Medical College, Hefei, 230061, Anhui, China. sxp20081012@sina.com.
Yajie WangDepartment of Pharmacy, Anhui Medical College, Hefei, 230061, Anhui, China.
Kaiwei KanDepartment of Pharmacy, Anhui Medical College, Hefei, 230061, Anhui, China.
Zhenbo ChaiDepartment of Pharmacy, Anhui Medical College, Hefei, 230061, Anhui, China.
Liping WangDepartment of Pharmacy, Anhui Medical College, Hefei, 230061, Anhui, China.
Mingyang LiDepartment of Pharmacy, Anhui Medical College, Hefei, 230061, Anhui, China.
Zhangqi LiuDepartment of Pharmacy, Anhui Medical College, Hefei, 230061, Anhui, China.

Funding

Anhui Provincial Natural Science Foundation 2022AH052316, GXXT-2022-002
6 · The paper itself

Abstract

To obtain a strain that efficiently secretes and expresses hlrGEL (recombinant human-like gelatin), and to establish a rapid method for detecting this expression, this study began with the original strain GS115/pPICZα-gel6 (4# clone). High-copy transformants were obtained through Zeocin resistance screening, and positive expression strains were rapidly screened using single-cell laser Raman spectroscopy (SCLRS) technology. The results revealed that the Raman spectral features of positive expressing strains were located at 1447 cm

Indexed as

GelatinRecombinant ProteinsSaccharomycetalesGene ExpressionHumansSpectrum Analysis, RamanGelatinRecombinant ProteinsGene copy numberHuman-like recombinant gelatinePichia pastoris (Komagataella phaffii)Single-cell laser raman spectroscopy

Identifiers

PMID40826158
PMCPMC12361465

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.