Evidence map›Paper›PMID 40824515›Full record

ArticleJournal of assisted reproduction and genetics2025

Normozoospermic seminal fluid extracellular vesicles could ameliorate adverse effects of freeze-thaw in oligoasthenoteratospermia men.

Roya Hassani, Hamid Reza Asgari, Morteza Koruji, Zahra Zandieh, Zohreh Nazmara

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Roya HassaniDepartment of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Hamid Reza AsgariStem Cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran. asgari.hr@iums.ac.ir.ORCID http://orcid.org/0000-0002-1864-0909
Morteza KorujiStem Cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran.
Zahra ZandiehDepartment of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Zohreh NazmaraDepartment of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Funding

Iran University of Medical Sciences IR.IUMS.FMD.REC.1402.076
6 · The paper itself

Abstract

backgroundCryopreservation can lead to sperm damage, which is why incorporating extracellular vesicles derived from normal semen can help protect against the damage caused by freezing. MATERIALS AND

methodsExtracellular vesicles were obtained from normozoospermic semen samples (n = 15), and their effects on the cryopreservation process were studied in three groups: the control group (A, n = 6), the extracellular vesicles treated before freezing group (B, n = 6), and the extracellular vesicles treated after freezing group (C, n = 6). Characteristics of sperm after thawing, including morphology, viability, motility, total antioxidant capacity (TAC), the rate of apoptosis, mitochondrial membrane potential (MMP), and the DNA fragmentation index (DFI), as well as the expression levels of the BAX, BCL

resultsGroup B displayed a significant increase in total motility, progressive motility, normal morphology, and MMP compared to groups A, and DNA fragmentation (DFI) showed a significant decrease compared to groups A and C. Also, vitality and TAC were significantly increased in group B compared to groups A and C. Decreased level of apoptosis was evident in group B compared to group A. Group A exhibited a significant increase in the expression of BAX/BCL

conclusionExtracellular vesicles obtained from normozoospermic seminal plasma could protect against the adverse cryopreservation effects through increased MMP and TAC, as well as decreased expression of BAX/BCL

Indexed as

AsthenozoospermiaCryopreservationExtracellular VesiclesOligospermiaSemenSemen PreservationSpermatozoaAdultApoptosisbcl-2-Associated X ProteinDNA FragmentationFreezingHumansMaleMembrane Potential, MitochondrialProto-Oncogene Proteins c-bcl-2bcl-2-Associated X ProteinProto-Oncogene Proteins c-bcl-2Extracellular vesiclesFreeze–thaw spermOligoasthenoteratospermia (OAT)Seminal fluid

Identifiers

PMID40824515
PMCPMC12640299

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.