ArticleJournal of assisted reproduction and genetics2025
Impact of repeated vitrification and biopsy on the developmental potential of blastocysts and clinical outcomes: A retrospective propensity-score-matched cohort study.
Article in Journal of assisted reproduction and genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Cumulative live birth following preimplantation genetic testing for aneuploidy versus conventional IVF/ICSI in unexplained recurrent pregnancy loss: a retrospective cohort study with intention-to-treat and per-protocol analyses.Frontiers in endocrinology · 2026Article
- Impact of PGT Introduction on IVF Laboratory Workload: Lessons Learned from a Single-Center Experience of 5258 Biopsies over a 10-Year Period.Life (Basel, Switzerland) · 2025Article
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Authors and funding
7 authors.
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Abstract
purposeThis study aimed to investigate the effects of repeated vitrification and trophectoderm (TE) biopsy procedures during preimplantation genetic testing (PGT) on blastocyst developmental potential and clinical outcomes.
methodsThis retrospective study analyzed data from January 2018 to January 2024. The study group included 229 blastocysts from 85 patients that underwent double vitrification (with either single or double biopsy). The control group consisted of 2,611 embryos from 654 patients who underwent conventional PGT with a single vitrification. Propensity score matching (PSM) in a 1:2 ratio was employed to balanced baseline characteristics. The primary outcome was live birth rate, with secondary outcomes including ploidy status, implantation, and miscarriage rates.
resultsAfter PSM, the double-vitrification group (226 blastocysts) was compared to a matched single-vitrification group (719 blastocysts). The double-vitrification group exhibited a significantly lower live birth rate (32.61% vs. 54.87%, p = 0.011). For embryos biopsied on Day 5, the double-vitrified group showed a significantly lower euploidy rate (37.80% vs. 48.79%, p = 0.032). A subgroup analysis within the double-vitrification group found no significant differences in ploidy status, clinical pregnancy rates, or live birth rates between embryos that underwent a single biopsy versus those that underwent a double biopsy (p > 0.05).
conclusionRepeated vitrification negatively impacts clinical outcomes in PGT cycles, specifically by reducing the live birth rate. However, the frequency of biopsy (single vs. double) does not appear to significantly affect clinical success. These findings suggest that while re-biopsy is a viable option for inconclusive cases, caution should be exercised regarding repeated vitrification procedures.
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