Evidence map›Paper›PMID 40824306›Full record

ArticleMolecular biology reports2025

Effect of lycium barbarum polysaccharide on ameliorating high-fat diet-damaged spermatogenesis via PCSK9 and TLR4 signaling pathways in obese mice.

Jinyuan Wang, Yuru Chen, Rongqian Ni, Mingxuan Su, Yichang Cao, Runtang Zhou, Xiang Li, Jiangming Li, Yuxia Tang, Jiayi Tang and 5 more

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In one paragraph

Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors.

Jinyuan Wang *Department of Reproduction and Genetics, The First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming, 650032, Yunnan Province, China.
Yuru Chen *Department of Reproduction and Genetics, The First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming, 650032, Yunnan Province, China.
Rongqian Ni *Department of Reproduction and Genetics, The First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming, 650032, Yunnan Province, China.
Mingxuan SuClinical Anatomy & Reproductive Medicine Application Institute, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, China.
Yichang CaoClinical Anatomy & Reproductive Medicine Application Institute, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, China.
Runtang ZhouClinical Anatomy & Reproductive Medicine Application Institute, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, China.
Xiang LiClinical Anatomy & Reproductive Medicine Application Institute, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, China.
Jiangming LiClinical Anatomy & Reproductive Medicine Application Institute, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, China.
Yuxia TangClinical Anatomy & Reproductive Medicine Application Institute, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, China.
Jiayi TangClinical Anatomy & Reproductive Medicine Application Institute, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, China.
Simin WangClinical Anatomy & Reproductive Medicine Application Institute, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, China.
Linyue TangClinical Anatomy & Reproductive Medicine Application Institute, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, China.
Ruxin ChenDepartment of Gynecological Endocrinology, Jinan Maternity and Child Care Hospital, Shandong First Medical University, Jianguo Xiaojing Road No. 2, Jinan, 250000, Shandong Province, China. crx0326@126.com.
Huawei WangDepartment of Reproduction and Genetics, The First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming, 650032, Yunnan Province, China. wanghuawei99@163.com.
Li TangDepartment of Reproduction and Genetics, The First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming, 650032, Yunnan Province, China. tanglikm@163.com.

Funding

National Natural Science Fund of China No. 82160281
6 · The paper itself

Abstract

backgroundObesity-induced chronic inflammation is a significant contributor to male infertility, primarily by disrupting Sertoli cell function and impairing spermatogenesis. Lycium barbarum polysaccharides (LBP), the principal bioactive constituents of wolfberries, exhibit a range of pharmacological effects, including anti-inflammatory, antioxidant, and anti-apoptotic activities.

objectiveHerein, we are committed to verifying whether LBP conferred inflammatory and apoptosis inhibition against obese spermatogenic dysfunction.

methodsPurified LBP was assessed for its effects on male fertility preservation in vivo. RNA-seq analysis was also employed to confirm the latent mechanism.

resultsIn vivo, We found that LBP significantly reversed lipid accumulation and regulated the secretion of inflammatory factors (MyD88, NF-κB, NLRP3), proliferation and apoptosis factors (Bax, Bcl2), and blood-testis barrier (BTB) markers (ZO-1, Ocln, CX43). This protective effect promoted Sertoli cell proliferation and improved semen quality in obese mice. The results showed that LBP treatment reduced cellular apoptosis. This was accompanied by increased expression of BTB-related proteins and restored anti-inflammatory activity. These findings suggest that PCSK9 and TLR4 accelerates Sertoli cell dysfunction under high-lipid conditions, while LBP intervention strongly reverses this dysfunction.

conclusionCollectively, these robust findings demonstrate that LBP alleviates high-fat diet-induced inflammatory responses and apoptosis by blocking the PCSK9 and TLR4 axis, improving BTB integrity and Sertoli cell dysfunction, and ultimately mitigating diet-induced male infertility.

Indexed as

Drugs, Chinese HerbalObesitySpermatogenesisToll-Like Receptor 4AnimalsApoptosisBlood-Testis BarrierCell ProliferationDiet, High-FatInfertility, MaleLyciumMaleMiceMice, Inbred C57BLMice, ObeseSertoli CellsDrugs, Chinese Herballycium barbarum polysaccharideTlr4 protein, mouseToll-Like Receptor 4InfertilityInflammationLycium barbarum polysaccharideObesitySertoli cells

Identifiers

PMID40824306

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.