Evidence map›Paper›PMID 40822576›Full record

ArticleProceedings of the West Virginia Academy of Science2025

Generation and comparison of broad-host range inducible expression vectors for use in Gram-negative bacteria including ESKAPE pathogens.

Raj K Ginde, Nicholas A Stella, Rachel C Calvario, Kara M Lehner, Jake D Callaghan, Daniel R Komlosi, Joseph Horzempa, Robert M Q Shanks

Abstract read
PubMed Full text at PMC
In one paragraph

Article in Proceedings of the West Virginia Academy of Science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Raj K GindeCharles T. Campbell Ophthalmic Microbiology Laboratory; Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, USA.
Nicholas A StellaCharles T. Campbell Ophthalmic Microbiology Laboratory; Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, USA.
Rachel C CalvarioCharles T. Campbell Ophthalmic Microbiology Laboratory; Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, USA.
Kara M LehnerCharles T. Campbell Ophthalmic Microbiology Laboratory; Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, USA.
Jake D CallaghanCharles T. Campbell Ophthalmic Microbiology Laboratory; Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, USA.
Daniel R KomlosiCharles T. Campbell Ophthalmic Microbiology Laboratory; Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, USA.
Joseph HorzempaDepartment of Biological Sciences, West Liberty University, West Liberty, WV, USA.
Robert M Q ShanksCharles T. Campbell Ophthalmic Microbiology Laboratory; Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, USA.

Funding

Virus Production and Manipulation of Protein/Gene Expression ModuleP30EY008098 · NEI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Ethan A Rossi · 1989 to 2026
$17.8M
Colistin-resistant Acinetobacter baumanniiR01AI104895 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI DOI, YOHEI, ERNST, ROBERT K · 2014 to 2024
$4.2M
Rise of lasR mutant Pseudomonas aeruginosa keratitisR01EY032517 · NEI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI SHANKS, ROBERT M · 2021 to 2024
$1.6M
Extensively drug resistant Pseudomonas aeruginosa keratitis outbreak strain:  virulence mechanisms and mitigationR01EY036686 · NEI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI ROBERT M SHANKS · 2024 to 2026
$1.4M
The mechanism and role of erythrocyte invasion by Francisella tularensisR15HL147135 · NHLBI · WEST LIBERTY UNIVERSITY · PI HORZEMPA, JOSEPH A · 2019 to 2023
$840k
NEI NIH HHS P30 EY008098NEI NIH HHS R01 EY032517NEI NIH HHS R01 EY036686NHLBI NIH HHS R15 HL147135NIAID NIH HHS R01 AI104895
6 · The paper itself

Abstract

The objective of this study is a controlled comparison of several carbohydrate inducible promoter in important bacterial species. Inducible promoter systems are invaluable for biotechnology and basic science applications. However, few inducible promoters are available on plasmids that replicate in

Indexed as

Acinetobacter baumanniiexpression vectorinducible promoterKlebsiella pneumoniaePseudomonasSerratia marcescens

Identifiers

PMID40822576
PMCPMC12352128

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.