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Background: Hydrothermal vent fields are habitats to a diverse array of benthic organisms, including several nematode species, which represent a significant portion of the biodiversity in these environments. Despite their ecological importance, most research on hydrothermal vents has focused on macro-invertebrates. As a result, vent nematode biodiversity remains largely unexplored, especially in peripheral and inactive structures, underscoring the need for further investigation. A sampling program conducted in 2017 and 2018 along a gradient of venting activity led to the collection of a number of Draconematidae species in various habitats. In this article, we introduce Methods: The samples were collected at the Lucky Strike vent field, on the Mid-Atlantic Ridge, using the suction sampler of the Remotely Operated Vehicle Victor6000. Specimens were retrieved from an edifice covered by a black layer of manganese oxy-hydroxides, with no local visible hydrothermal activity, at a depth of 1.639 m. Samples were sieved on a 32 µm mesh onboard, sorted and, for nematodes, identified to species level back in the lab. Fluorescent images were obtained using the ApoTome Fluorescence Microscope Module, and 3D observations were possible through the depth change method. Results: We established Conclusion: This discovery contributes to the knowledge of Draconematidae biodiversity, and highlights the importance to investigate nematode communities at species-level, data that is often missing at vent studies. Additionally, it underscores the significance of preserving inactive hydrothermal habitats, which are threatened by deep-sea mining activities.