Evidence map›Paper›PMID 40821988›Full record

ArticlePeerJ2025

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Samuel I Koehler, Earl A Middlebrook, Blake T Hovde, Erik R Hanschen

Abstract read
In one paragraph

Article in PeerJ, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Samuel I KoehlerBioscience Division, Los Alamos National Laboratory, Los Alamos, NM, United States of America.
Earl A MiddlebrookBioscience Division, Los Alamos National Laboratory, Los Alamos, NM, United States of America.
Blake T HovdeBioscience Division, Los Alamos National Laboratory, Los Alamos, NM, United States of America.
Erik R HanschenBioscience Division, Los Alamos National Laboratory, Los Alamos, NM, United States of America.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microbiology research was conducted for decades before widespread availability of sequencing resources and large culture collection sequence repositories, making it challenging to efficiently identify and validate strains used in historical studies. Similarly, finding commercially available microbe strains similar to strains of interest, or containing target genes of interest found during metagenomic experiments is challenging. Despite tremendous advances in sequencing data availability, database curation, and sequence-searching software capabilities, identifying commercially available microbe strains from sequence data remains complicated and tedious. The American Type Culture Collection (ATCC) is an organization selling a wide variety of microbes, uniquely providing strain-level taxonomy classification and associated sequenced reference genomes for over four thousand isolates, with more being added regularly. As researchers purchase and sequence isolates from ATCC, many sequences derived from ATCC isolates are deposited on public databases such as NCBI-Genome. Sequences uploaded to public databases will vary in laboratory, bioinformatics, and metadata quality and can also contain mutations derived from cultivation which are not representative of ATCC stocks. Using ATCC-sourced reference genomes ensures consistent quality and analysis methodologies are implemented to accurately represent strain sequences. Currently, ATCC does not provide methods to search for sequence similarity between many query sequences and ATCC genomes. While NCBI-BLAST could be used to search for queries against GenBank, with results filtered for "ATCC" tags, search result quality varies and requires time-consuming sorting. Here we present the software

Indexed as

AlgorithmsComputational BiologyDatabases, Nucleic AcidGenome, MicrobialMicrobiologyGenetics, MicrobialSoftware ValidationATCCATCC genome portalMicrobe culturingMicrobe identificationSequence querySoftware

Identifiers

PMID40821988
PMCPMC12357551

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.