ArticleAmerican journal of translational research2025
Huatan Tongmai Yin regulates RFRP-3 to intervene in the "microbe-gut-HPO axis" to treat PCOS-IR rats.
Article in American journal of translational research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- The Gut-Reproduction-Oral-Immune Axis: Microbial Dysbiosis as a Shared Driver in Polycystic Ovary Syndrome and Endometriosis.Current microbiology · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo explore the therapeutic effect of Huatan Tongmai Decoction on insulin-resistant polycystic ovary syndrome (PCOS-IR) and the potential mechanism.
methodsThe PCOS-IR rat model was constructed and HE staining was used to observe the morphological changes of vaginal shedding cells and ovary. Serum sex hormone levels, fasting insulin (FINS), fasting blood glucose (FBG) and inflammatory factors were detected by enzyme-linked immunosorbent assay (ELISA). Intestinal flora was analyzed by 16SrDNA sequencing. The mRNA expression of RFRP-3 and GPR147 in hypothalamus tissues was detected by RT-qPCR. The expression of ZO-1 and Occludin in colon tissue and the expression of GPR54, MyD88, TLR4, GnRHR and KP in the hypothalamus were detected by Western blot.
resultsCompared with the model group, the body weight of the Huatan Tongmai Yin group decreased, the estrus cycle returned to normal, the levels of serum T, LH, FINS, HOMA-IR and inflammatory factors decreased, the levels of FSH increased, the types of intestinal flora OTU were more abundant, ACE and Chao index increased, and the mRNA level of hypothalamus RFRP-3 was up-regulated. In addition, serum T, LH, FPG, FINS and HOMA-IR levels were decreased, FSH levels were increased, inflammatory factors were down-regulated, and hypothalamic GPR54, MyD88, TLR4 and GnRHR proteins were reduced in rats after overexpression of RFRP-3.
conclusionTantongmai Decoction can correct the imbalance of intestinal flora, improve intestinal mucosal barrier function, relieve chronic inflammation and insulin resistance, thus up-regulating RFRP-3 in the hypothalamus, inhibiting the release of GnRH and KP, reducing the secretion of LH and T, and effectively alleviating PCOS-IR by regulating "microbe-gut-HPO axis".
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.