ArticleAmerican journal of translational research2025
Spheroid culture of human periodontal ligament stem cells on agarose enhances stemness and osteogenic differentiation potential.
Article in American journal of translational research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Palm Mixed-Carotenes Modulate Viability, Wound Closure and Osteoprotegerin mRNA Expression in Human Periodontal Ligament Stem Cells.Biomedicines · 2026Article
- Diosmin-Capped Silver Nanoparticles Promote Osteogenic Differentiation of Human Periodontal Ligament Stem Cells: Box-Behnken Optimization and in vitro Evaluation.International journal of nanomedicine · 2026Article
- Pulsed electromagnetic field stimulation subtly modulates doxorubicin sensitivity in a spheroid co-culture model of osteosarcoma.Scientific reports · 2025Article
- Bioengineering of Periodontal Tissues: Cell Therapy and Biomaterials Application.Bioengineering (Basel, Switzerland) · 2025Review
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Authors and funding
10 authors.
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Abstract
objectiveTo develop an agarose (AG)-based spheroid culture system to enhance the stemness and osteogenic potential of human periodontal ligament stem cells (hPDLSCs), addressing limitations of current periodontal tissue engineering approaches.
methodshPDLSCs were isolated from extracted premolars and cultured as spheroids in AG-coated 96-well plates. Spheroid formation was optimized by varying cell seeding densities and culture durations. Monolayer-cultured hPDLSCs served as controls. Stemness was evaluated using RT-qPCR and immunofluorescence detection of pluripotency markers. Cell migratory capacity was assessed by scratch assays. Osteogenic differentiation was induced for 10-21 days, and calcium deposition was quantified by Alizarin Red S staining.
resultsSpheroid-derived hPDLSCs showed significantly higher expression of stem cell markers (OCT4 and NANOG) and greater migratory capacity compared to monolayer-cultured cells. Upon osteogenic induction, spheroid-derived hPDLSCs exhibited upregulated expression of osteogenesis-related genes (BSP and OPN) and formed more extensive calcium nodules than their monolayer counterparts.
conclusionThe AG-based spheroid culture system effectively maintains hPDLSC stemness and enhances their osteogenic differentiation potential. This scalable and cost-effective platform provides high-quality seed cells for periodontal regeneration and holds promise for improving clinical outcomes in periodontal therapy.
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