Evidence map›Paper›PMID 40820584›Full record

ArticleCNS neuroscience & therapeutics2025

MicroRNA-21a-5p Promotes Cerebral Angiogenesis in Transient Ischemic Attack by Targeting RBMS3 and Subsequently Modulating the TGFBR1/SMAD2/3 Pathway.

Jiahui Wang, Yanyan Li, Jingyi Wang, Shiling Chen, Luwei Nie, Xuan Wu, Jiarui Li, Ping Zhang, Zhouping Tang

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In one paragraph

Article in CNS neuroscience & therapeutics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jiahui WangDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Yanyan LiDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Jingyi WangDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Shiling ChenDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Luwei NieDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Xuan WuDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Jiarui LiDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Ping ZhangDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Zhouping TangDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.ORCID 0000-0002-4153-8590

Funding

Interdisciplinary Research Program of Huazhong University of Science and Technology 2024JCYJ044Key Research and Development Program of Wuhan 2024020702030123Major Program (JD) of Hubei Province 2023BAA005National Nature Science Foundation of China 92148206Research Fund of Tongji Hospital 2022ZHFY01Research Fund of Tongji Hospital AI2024B03
6 · The paper itself

Abstract

aimsTransient ischemic attack (TIA) serves as a crucial precursor to a potential stroke. Angiogenesis is essential for the renovation of the damaged brain after TIA. This study aimed to elucidate the role of miRNA-21a-5p in angiogenesis following TIA and unravel the underlying mechanisms.

methodsIn vivo and in vitro TIA models were established using the modified suture middle cerebral artery occlusion and oxygen-glucose deprivation/reoxygenation methods. Differentially expressed miRNAs in the TIA group versus the control group were identified using small RNA sequencing. The putative target genes of miRNA-21a-5p were predicted via bioinformatics analysis and validated using a dual luciferase reporter gene assay. TIA models were then treated with miRNA-21a-5p antagomir/agomir or/and an adeno-associated virus interfering with the target gene to assess the effects of miRNA-21a-5p and the identified target gene on angiogenesis after TIA.

resultsMiRNA-21a-5p was the miRNA with the most significant changes after TIA. RBMS3 was identified as a target gene for miRNA-21a-5p. Downregulation of miRNA-21a-5p expression reduced angiogenesis in animal and cellular models of TIA, and miRNA-21a-5p upregulation had a completely opposite effect. RBMS3 suppression reversed miRNA-21a-5p knockdown-mediated inhibition of angiogenesis in TIA models. Moreover, the TGFBR1/SMAD2/3 pathway was found to be downstream for miRNA-21a-5p/RBMS3.

conclusionMiRNA-21a-5p conferred neuroprotective effects against TIA by enhancing angiogenesis through the RBMS3/TGFBR1/SMAD2/3 pathway.

Indexed as

Ischemic Attack, TransientMicroRNAsReceptor, Transforming Growth Factor-beta Type ISmad2 ProteinAngiogenesisAnimalsHumansInfarction, Middle Cerebral ArteryMaleRatsRats, Sprague-DawleySignal TransductionSmad3 ProteinMicroRNAsmirn21 microRNA, ratReceptor, Transforming Growth Factor-beta Type ISmad2 ProteinSmad2 protein, ratSmad3 ProteinSmad3 protein, ratTgfbr1 protein, ratangiogenesismiRNA‐21a‐5pRBMS3TGFBR1transient ischemic attack

Identifiers

PMID40820584
PMCPMC12358734

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.