ArticleScientific reports2025
Impact of cryopreservation agents on sperm quality, DNA fragmentation, and apoptotic markers in fertile and infertile males.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Sperm DNA fragmentation and its influence on mammalian reproduction.Nature reviews. Urology · 2026Review
- Article
- Protective Effect of Intracellular Compounds of Lactobacillus plantarum on Goat Sperm Quality During Cryopreservation.Veterinary medicine and science · 2026Article
- Review
- Age-Associated Alterations in Sperm Quality and DNA Integrity in Cryopreserved Bali Bulls (Veterinary medicine international · 2026Article
- Cryopreservation increases sperm DNA fragmentation in normozoospermic Libyan men: the role of oxidative stress and the protective effect of melatonin.The Libyan journal of medicine · 2025Article
- Semen Quality in Patients with Hematological Malignancies: A Retrospective Monocentric Study in the Perspective of Personalized Oncofertility Medicine.Journal of personalized medicine · 2025Article
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5 authors.
Funding
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Abstract
Semen cryopreservation is a crucial technique for preserving male fertility, playing a vital role in assisted reproductive procedures by storing frozen semen samples for artificial insemination (AI) and intra-cytoplasmic sperm injection (ICSI) to enhance reproductive success rates. This study aims to identify the most effective cryopreservation methods and assess their impact on semen quality, particularly sperm DNA fragmentation. A total of 30 semen samples were categorized into fertile and infertile groups. DNA fragmentation analysis was conducted using the Sperm Chromatin Structure Assay (SCSA). Each sample was divided into three portions and frozen using different cryoprotectants: (egg-yolk + glycerol), (sucrose + glycerol), and (glycerol alone). After one month of storage, the samples were analyzed to determine the most effective medium. The findings revealed a decline in sperm motility post-freezing compared to fresh samples, along with a slight increase in morphological abnormalities. Additionally, there was a rise in sperm DNA fragmentation and an increase in apoptotic marker (Caspase-3) levels after the freezing process. The study concluded that cryopreservation and thawing caused some degree of sperm cell damage, with infertile samples being more adversely affected than fertile ones.
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