Evidence map›Paper›PMID 40818764›Full record

ArticleBiomedical journal2026

Systematic analysis of mushroom body-innervating dopaminergic neuron activity in different physiological states in Drosophila.

Peng-Shiuan Lee, Meng-Hsuan Chiang, Tony Wu, Chia-Lin Wu

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Article in Biomedical journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

What it found

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Peng-Shiuan LeeDepartment of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Meng-Hsuan ChiangGraduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Department of Biochemistry, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Tony WuDepartment of Neurology, New Taipei Municipal TuCheng Hospital, Chang Gung Memorial Hospital, New Taipei City, Taiwan.
Chia-Lin WuGraduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Department of Biochemistry, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Department of Neurology, New Taipei Municipal TuCheng Hospital, Chang Gung Memorial Hospital, New Taipei City, Taiwan; Brain Research Center, National Tsing Hua University, Hsinchu, Taiwan. Electronic address: clwu@mail.cgu.edu.tw.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThirst and hunger are fundamental survival drives that modulate various aspects of animal behavior through specific neural circuits. Previous studies have demonstrated that dopaminergic neurons (DANs) innervating the mushroom body (MB) in the Drosophila brain play essential roles in innate and learned thirst- and hunger-dependent behaviors, with most experiments focusing on acute water or food deprivation. However, it is unclear whether acute water or food deprivation alters dopamine production and neural activity in MB-innervating DANs. MATERIAL AND

methodsWe genetically expressed green fluorescent protein (GFP) in MB-innervating DANs using broadly and specifically labeled GAL4 lines under satiety, thirst, and hunger states. The brains were immunostained with anti-tyrosine hydroxylase (TH) to assess dopamine biosynthesis. Additionally, the transcriptional reporter of intracellular Ca

resultsNeither TH levels nor neural activity in the 13 subtypes of MB-innervating DANs exhibited significant differences during the satiety, thirst, and hunger conditions.

conclusionThis study suggests that 16-h water deprivation or 24-h food deprivation does not significantly alter dopamine production and neural activity in MB-innervating DANs. These findings offer insights into the independence of baseline dopaminergic activity from internal states in thirst- or hunger-related behaviors.

Indexed as

Dopaminergic NeuronsDrosophilaMushroom BodiesAnimalsDopamineDrosophila melanogasterFood DeprivationHungerThirstTyrosine 3-MonooxygenaseDopamineTyrosine 3-MonooxygenaseDopaminergic neuronHungerMushroom bodyNeural activityThirstTyrosine hydroxylase

Identifiers

PMID40818764
PMCPMC13226800

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.