ArticleNeurogastroenterology and motility2025
Phosphorylation of Pyruvate Dehydrogenase as a Marker of Neuronal Inactivity in the Enteric Nervous System.
Article in Neurogastroenterology and motility, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundThe enteric nervous system (ENS) regulates essential gut functions through interactions between neurons and glial cells. While tools for studying neuronal activation are well-established, methods for tracking neuronal inactivation remain underdeveloped. Phosphorylated pyruvate dehydrogenase (pPDH) has emerged as a marker of neuronal inactivity in the brain. This study investigates whether pPDH can similarly serve as a reliable marker of neuronal inactivity in the ENS.
methodsWhole-mount preparations of the colonic myenteric plexus from mice were treated with veratridine (neuronal activator) or tetrodotoxin (neuronal inhibitor). Immunohistochemistry was used to label neurons with HuC/D and quantify pPDH (inactivity marker) and pERK (activation marker) labelling. Fluorescent signals were analyzed to assess changes in marker expression under different conditions.
resultsTTX treatment increased pPDH labelling, as evidenced by higher fluorescence intensity and a greater percentage of pPDH-positive neurons. In contrast, veratridine reduced pPDH levels, indicating its sensitivity to neuronal activity changes. Together, pPDH and pERK provide reliable measures of neuronal inactivation and activation, respectively, in the ENS.
conclusionsThis study establishes pPDH as a robust marker for neuronal inactivation in the ENS, complementing existing activation markers like pERK. These findings provide a novel framework for exploring neuron-glia communication and neuronal dynamics in the gut.
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