ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025
Assessing Extracellular Vesicle Turnover In Vivo Using Highly Sensitive Phosphatidylserine-Binding Reagents.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed.
- A Beginner's Guide to Study the Role of Extracellular Vesicles in Coagulation.Journal of extracellular biology · 2026Review
- Loss of Atp8a2 drives neurodegeneration through the dysregulation of spatiotemporal phosphatidylserine externalization in mature neurons.Cell death & disease · 2026Article
- Brain targeting and trafficking of extracellular vesicles in central nervous system diseases: a therapeutic roadmap.Nanomedicine (London, England) · 2026Review
- Peculiarities of Phosphatidylserine Externalization by Nano- and Microsecond Electric Pulses.The Journal of membrane biology · 2026Article
- Review
- The Dual Role of Extracellular Vesicles in Aging and Age-Related Diseases: Pathophysiology and Therapeutic Potential.International journal of nanomedicine · 2026Review
- Circulating extracellular vesicle-microRNAs (EV-miRNAs) in leukemias and related disorders.Frontiers in medicine · 2026Review
- Assessing Extracellular Vesicle Turnover In Vivo Using Highly Sensitive Phosphatidylserine-Binding Reagents.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Extracellular vesicles (EVs) are emerging as crucial players in cell communication and hold great promise as biomarkers and therapeutic tools. However, their diversity makes it challenging to detect, classify, and utilize them effectively, which limits their clinical applicability. A key challenge is the lack of reliable markers to identify EVs consistently. In this study, a novel high-affinity phosphatidylserine (PS)-binding reagent is introduced for EV analysis. PS is known as a marker of apoptotic cells and activated platelets, but its presence on EVs is debated due to variations in lipid composition. By comparing multiple PS-binding reagents, including MFG-E8 derivatives and Annexin V, it is demonstrated that ≈90% of EVs in human and mouse blood carry PS. Using the optimized reagent, the first in vivo insights into EV turnover are provided, showing that PS+ EVs in mouse blood are rapidly cleared (≈50% within 30 min) but persist on immune cells in the spleen. This discovery increases the potential of EVs as disease biomarkers and therapeutic targets by improving EV detection and isolation as well as opening the door for standardized quantification and diagnostic monitoring.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.