ArticleBMC cancer2025
HACD3 promotes malignant progression of NSCLC by suppressing the MKK7/MAPK10 signaling axis.
Article in BMC cancer, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundHACD3 is from the very long-chain fatty acid dehydratase family and exhibits only mild activity while producing long-chain fatty acids. Numerous studies have uncovered an association of HACD3 with the advancement of diverse cancers. The current study attempts to delve into the potential role of HACD3 in initiating and enhancing lung cancer.
objectiveThis project aims to investigate the role of HACD3 in the malignant progression of lung cancer and its related mechanisms.
methodsHACD3 expression was assessed in NSCLC tissues, cell lines, and GEPIA and HPA public databases. Functional consequences of HACD3 knockdown and overexpression were examined in vitro by assessing cell proliferation, colony formation, migration, cell cycle progression, and apoptosis, and in vivo through assays for xenograft tumorigenesis in NSG mice. Systemic Hacd3 knockout (Hacd3
resultsHACD3 was found significantly overexpressed in NSCLC tissues and cells. Increased HACD3 levels promoted key malignant behaviors including proliferation and migration in vitro, and accelerated tumor growth in vivo. Genetic deletion of Hacd3 markedly reduced lung tumor formation in a urethane model, an effect independent of major changes to fatty acid metabolism. Mechanistic studies revealed that HACD3 suppresses the MAPK pathway via direct physical interaction with MKK7 and MAPK10. Finally, the core pro-tumorigenic activity was mapped to a specific C-terminal domain encompassing amino acids 231-259.
conclusionsHACD3 promotes the malignant progression of NSCLC by interacting with MKK7 and MAPK10 to suppress MAPK signaling, independent of its canonical dehydratase activity. The identification of a critical pro-oncogenic domain (aa231-259) suggests that targeting HACD3, potentially via this region, represents a novel therapeutic strategy for NSCLC.
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