Evidence map›Paper›PMID 40816273›Full record

ArticleStem cell reports2025

Germline transmission of cryopreserved mouse spermatogonial stem cells maintained on the International Space Station.

Mito Kanatsu-Shinohara, Takuya Yamamoto, Yusuke Shiromoto, Hiroko Morimoto, Tianjiao Liu, Tohru Yamamori, Tomokazu Yamasaki, Takashi Shinohara

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mito Kanatsu-ShinoharaDepartment of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan; AMED, 1-7-1 Otemachi, Chiyodaku, Tokyo 100-0004, Japan.
Takuya YamamotoDepartment of Life Science Frontiers, Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan; Institute for the Advanced Study of Human Biology (WPI-ASHIBi), Kyoto University, Yoshida-Konoe, Sakyo, Kyoto 606-8501, Japan; Medical-risk Avoidance based on iPS Cells Team, RIKEN Center for Advanced Intelligence Project (AIP), Kyoto 606-8507, Japan.
Yusuke ShiromotoDepartment of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
Hiroko MorimotoDepartment of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
Tianjiao LiuDepartment of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
Tohru YamamoriSpace Utilization Promotion Department, Japan Space Forum, Tokyo 101-0062, Japan.
Tomokazu YamasakiJapan Aerospace Exploration Agency, Tsukuba 305-8505, Japan.
Takashi ShinoharaDepartment of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan. Electronic address: tshinoha@virus.kyoto-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Despite growing interest in space exploration, the effects of spaceflight on stem cells remain largely unknown. Damages to germline cells are especially crucial, as spaceflight poses risks to gametogenesis, with abnormalities observed in multiple species. While embryonic stem (ES) cells show genomic instability after space exposure and have not yet produced offspring, this study demonstrates successful offspring production from cryopreserved mouse spermatogonial stem cells (SSCs) stored on the International Space Station for 6 months. Spaceflight did not increase apoptosis or DNA damage in SSCs. After thawing, SSCs proliferated comparably to those cryopreserved on Earth, showing no significant phenotypic or functional differences. Offspring were produced via spermatogonial transplantation followed by natural mating. Since SSCs from many species can be cryopreserved like somatic cells and still produce sperm, they offer a promising resource for germline preservation during space exploration.

Indexed as

Adult Germline Stem CellsCryopreservationSpace FlightSpermatogoniaAnimalsApoptosisDNA DamageFemaleMaleMicecryopreservationspaceflightspermatogonial stem cellstransplantation

Identifiers

PMID40816273
PMCPMC12447337

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.