Evidence map›Paper›PMID 40814003›Full record

ArticleVeterinary research2025

Development and characterization of monoclonal antibodies specific for bovine IP-10.

Hamza Khalid, Michael Coad, Inga Dry, Catherine McGuinnes, Lindsey A Waddell, Jayne C Hope, Zhiguang Wu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Hamza KhalidDivision of Immunology, The Roslin Institute, University of Edinburgh, Edinburgh, EH25 9RG, UK.
Michael CoadAnimal and Plant Health Agency, Weybridge, Surrey, KT15 3NB, UK.
Inga DryDivision of Immunology, The Roslin Institute, University of Edinburgh, Edinburgh, EH25 9RG, UK.
Catherine McGuinnesDivision of Immunology, The Roslin Institute, University of Edinburgh, Edinburgh, EH25 9RG, UK.
Lindsey A WaddellDivision of Immunology, The Roslin Institute, University of Edinburgh, Edinburgh, EH25 9RG, UK.
Jayne C HopeDivision of Immunology, The Roslin Institute, University of Edinburgh, Edinburgh, EH25 9RG, UK.
Zhiguang WuDivision of Immunology, The Roslin Institute, University of Edinburgh, Edinburgh, EH25 9RG, UK. zhiguang.wu@roslin.ed.ac.uk.ORCID http://orcid.org/0000-0001-9131-730X

Funding

Animal Health and Veterinary Laboratories Agency SE3334Biotechnology and Biological Sciences Research Council BB/CCG2270/1Biotechnology and Biological Sciences Research Council BBS/E/D/20002174Biotechnology and Biological Sciences Research Council BBS/E/RL/230002B
6 · The paper itself

Abstract

Research on chemokines in cattle is hampered by the relative lack of validated reagents. Bovine IP-10 is an important inflammatory chemokine and a promising diagnostic biomarker for an economically important disease (bovine tuberculosis) caused by infection of cattle with Mycobacterium bovis. Currently no monoclonal antibodies are available for bovine IP-10. The goal of this study was to generate novel mAbs for detection of bovine IP-10 using hybridoma technology. Five mAbs were developed and cross clone inhibition analyses showed a high degree of self-inhibition among the mAbs. One mAb (7C2) was used in conjugation with a commercial polyclonal antibody to develop a sandwich ELISA. Upon testing with recombinant bovine IP-10, this ELISA showed an enhanced linear range compared to the currently available polyclonal antibody-based ELISA. The ELISA using 7C2 was shown to detect native antigen-specific bovine IP-10 in samples from M. bovis infected animals. The 7C2 mAb could also detect intracytoplasmic IP-10. These novel mAbs will be useful in elucidating roles for IP-10 in bovine immune studies in health, disease and vaccination contexts.

Indexed as

Antibodies, MonoclonalChemokine CXCL10Mycobacterium bovisTuberculosis, BovineAnimalsCattleEnzyme-Linked Immunosorbent AssayHybridomasAntibodies, MonoclonalChemokine CXCL10bovine tuberculosishybridoma technologyintracellular stainingIP-10monoclonal antibody

Identifiers

PMID40814003
PMCPMC12351912

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.