Evidence map›Paper›PMID 40813949›Full record

ArticleDiscover oncology2025

High expression of LIMD2 predicts a poor prognosis and promotes migration of colon cancer cells.

Jianjun Wang, Fengjuan Cai, Guodong Liu, Jingtao Zhang, Dongdong Li, Yujie Cai, Yu Wang, Wanjun Xu, Xiaoyan Wang, Xiaomin Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Discover oncology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jianjun Wang *Department of Histology and Embryology, Wannan Medical College, Wuhu, 241002, Anhui, China.
Fengjuan Cai *Department of Gastroenterology, Clinical Medical Research Center, Department of General Surgery, Jiangsu Provincial People's Hospital Suqian Hospital, The Suqian Clinical College of Xuzhou Medical University, Suqian Clinical Medical College of Jiangsu University, Suqian, 223800, Jiangsu, China.
Guodong Liu *Department of Gastroenterology, Clinical Medical Research Center, Department of General Surgery, Jiangsu Provincial People's Hospital Suqian Hospital, The Suqian Clinical College of Xuzhou Medical University, Suqian Clinical Medical College of Jiangsu University, Suqian, 223800, Jiangsu, China.
Jingtao ZhangDepartment of Gastroenterology, Clinical Medical Research Center, Department of General Surgery, Jiangsu Provincial People's Hospital Suqian Hospital, The Suqian Clinical College of Xuzhou Medical University, Suqian Clinical Medical College of Jiangsu University, Suqian, 223800, Jiangsu, China.
Dongdong LiDepartment of Gastroenterology, Clinical Medical Research Center, Department of General Surgery, Jiangsu Provincial People's Hospital Suqian Hospital, The Suqian Clinical College of Xuzhou Medical University, Suqian Clinical Medical College of Jiangsu University, Suqian, 223800, Jiangsu, China.
Yujie CaiDepartment of Gastroenterology, Clinical Medical Research Center, Department of General Surgery, Jiangsu Provincial People's Hospital Suqian Hospital, The Suqian Clinical College of Xuzhou Medical University, Suqian Clinical Medical College of Jiangsu University, Suqian, 223800, Jiangsu, China.
Yu WangDepartment of Gastroenterology, Clinical Medical Research Center, Department of General Surgery, Jiangsu Provincial People's Hospital Suqian Hospital, The Suqian Clinical College of Xuzhou Medical University, Suqian Clinical Medical College of Jiangsu University, Suqian, 223800, Jiangsu, China.
Wanjun XuLaboratory of Clinical and Experimental Pathology, National Demonstration Center for Experimental Basic Medical Science Education, School of Basic Medical Sciences, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
Xiaoyan WangDepartment of Gastroenterology, Clinical Medical Research Center, Department of General Surgery, Jiangsu Provincial People's Hospital Suqian Hospital, The Suqian Clinical College of Xuzhou Medical University, Suqian Clinical Medical College of Jiangsu University, Suqian, 223800, Jiangsu, China. 17805296777@163.com.
Xiaomin LiDepartment of Gastroenterology, Clinical Medical Research Center, Department of General Surgery, Jiangsu Provincial People's Hospital Suqian Hospital, The Suqian Clinical College of Xuzhou Medical University, Suqian Clinical Medical College of Jiangsu University, Suqian, 223800, Jiangsu, China. lxm1980326@sina.com.

Funding

Guangdong Basic and Applied Basic Research Foundation 2021A1515010665Key project fund of Jiangsu Provincial Health Commission ZD2022052National Natural Science Foundation of China 81902479Natural Science Foundation of the Anhui provincial High-Education Institutions of China KJ2021A0828Suqian Sci&Tech Program KY202304Suqian Talent Xiongying Plan Project SQXY202436The Social Development Projects of Key R&D Programs in Xuzhou city KC23247
6 · The paper itself

Abstract

backgroundLIM domain-containing protein 2 (LIMD2) is known to promote metastasis in several cancers. However, its role and underlying mechanisms in colon cancer remain unclear. This study focused on investigating the prognostic value, functional impact, and molecular mechanisms of LIMD2 in colon cancer.

methodsLIMD2 expression in colon cancer tissues and matched non-cancerous tissues was detected using RT-qPCR and immunohistochemistry. The chi-square test was used to assess the association between LIMD2 expression and clinicopathological characteristics. Kaplan-Meier survival analysis and Cox proportional hazards models were applied to evaluate the prognostic value of LIMD2. The functional role of LIMD2 in colon cancer cell migration was examined through Transwell and wound healing assays. Additionally, RNA sequencing (RNA-seq), co-immunoprecipitation (CoIP), silver staining, and mass spectrometry were performed to uncover the role of LIMD2 in colon cancer cell migration.

resultsLIMD2 expression was significantly increased in colon cancer samples and cell lines. High LIMD2 expression was positively correlated with lymph node metastasis and TNM stage. Patients with elevated LIMD2 expression exhibited poorer overall survival (OS). Gain-of-function assays demonstrated that LIMD2 accelerated colon cancer cell migration. RNA-seq analysis revealed that LIMD2 regulates ECM-receptor interactions and focal adhesion pathways. Furthermore, CoIP and mass spectrometry identified cofilin1 as a LIMD2-interacting protein.

conclusionsOur findings indicate that LIMD2 serves as a novel prognostic biomarker and potential target for colon cancer treatment.

Indexed as

Colon cancerLIMD2MigrationPrognosis

Identifiers

PMID40813949
PMCPMC12354938

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.