Evidence map›Paper›PMID 40813407›Full record

ArticleScientific reports2025

Effect of ultrasound-microbubble exposure on acute myeloid leukemia cancer cell proteome.

Joelle Stilwell, Daron A Savaya, Emmanuel Sakarya, Charlotte Ferworn, Eleanor Cloves, Roberto J Botelho, Costin N Antonescu, Raffi Karshafian

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Joelle StilwellDepartment of Physics, Toronto Metropolitan University, 350 Victoria Street, Toronto, ON, M5B 2K3, Canada.
Daron A SavayaDepartment of Physics, Toronto Metropolitan University, 350 Victoria Street, Toronto, ON, M5B 2K3, Canada.
Emmanuel SakaryaDepartment of Physics, Toronto Metropolitan University, 350 Victoria Street, Toronto, ON, M5B 2K3, Canada.
Charlotte FerwornDepartment of Physics, Toronto Metropolitan University, 350 Victoria Street, Toronto, ON, M5B 2K3, Canada.
Eleanor ClovesDepartment of Chemistry and Biology, Toronto Metropolitan University, Toronto, ON, Canada.
Roberto J BotelhoDepartment of Chemistry and Biology, Toronto Metropolitan University, Toronto, ON, Canada.
Costin N AntonescuDepartment of Chemistry and Biology, Toronto Metropolitan University, Toronto, ON, Canada.
Raffi KarshafianDepartment of Physics, Toronto Metropolitan University, 350 Victoria Street, Toronto, ON, M5B 2K3, Canada. karshafian@torontomu.ca.

Funding

Natural Sciences and Engineering Research Council (NSERC) Discovery grants program RGPIN-201505941
6 · The paper itself

Abstract

The application of ultrasound in combination with microbubbles (USMB) induces a broad spectrum of bioeffects on cells including plasma membrane disruption, cytoskeleton rearrangement, influx/efflux of cytosolic/extracellular molecules, metabolic stress and signalling pathways. This study investigated the effect of USMB on cell proteomics in human acute myeloid leukemia cells. Cells (OCI-AML-5) in suspension were exposed to ultrasound (f = 1 MHz, PD = 20 μs and PRP = 1 ms for 120 s) with and without microbubbles (Definity at 1.7% v/v) at varying acoustic pressures. Following exposure, cells were analysed using flow cytometry, mass spectrometry and immunoblotting. USMB enhanced cell membrane permeability (~ 37%), as expected, and induced significant changes in the cell proteome. Of 6825 proteins, 78 (~ 1.14%) were statistically significant and had a fold-change (≤ 0.8 or ≥ 1.2) compared to untreated control, referred to as a differentially expressed protein (DEP). The highest fold change of ~ 4-5 was induced in HMOX1 protein. The protein expression depended on acoustic pressure, presence of microbubbles and elapsed-time following exposure to ultrasound. Western blotting analysis confirmed the effect of USMB on three proteins (HMOX1, BACH1 and ANXA1) with R

Indexed as

Leukemia, Myeloid, AcuteMicrobubblesProteomeUltrasonic WavesCell Line, TumorCell Membrane PermeabilityHumansProteomicsProteomeAcute myeloid leukemiaMicrobubbleProteomeSonoporationUltrasound therapy

Identifiers

PMID40813407
PMCPMC12354902

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.