ArticleThe Journal of nutrition2025
Vitamin B-6 Deficiency Induces Anxiety-Like Behavior in Sprague-Dawley Rats by Disrupting Gut Homeostasis.
Article in The Journal of nutrition, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVitamin B-6 (VB-6) is essential for numerous metabolic pathways, including neurotransmitter synthesis, and its deficiency has been linked to neurologic disorders. Emerging evidence suggests that B vitamin deficiencies can disturb the gut microbiome. Although the gut-brain axis is well recognized, the influence of VB-6 deficiency on behavior via gut-mediated mechanisms remains poorly understood.
objectivesThis study aimed to investigate the role of VB-6 in modulating the gut-brain axis and its impact on neurobehavioral outcomes.
methodsSixty-four Sprague-Dawley rats were fed an AIN-93G-based diet with either optimal [optimum vitamin B-6 (OB-6)] or deficient [deficient vitamin B-6 (DB-6)] amounts of VB-6 for 7 wk. Half of the rats received antibiotics in drinking water (ampicillin, 1 g/L; metronidazole, 1 g/L; and neomycin, 1 g/L) plus weekly gavage (vancomycin, 500 mg/L, and amphotericin B, 0.1 mg/mL). Plasma VB-6, neurotransmitters, short-chain fatty acids (SCFAs), behavioral assessments, gut microbiome composition, and gut histology were assessed. Data were analyzed using 2-way or 3-way analysis of variance with Fisher's least significant difference post hoc test (P ≤ 0.05).
resultsDB-6 rats showed ≤96% reduction in plasma VB-6 (P < 0.001), 22% decrease in brain γ-aminobutyric acid (P < 0.01), and 32% increase in glutamic acid (P < 0.01) compared with OB-6 animals. In the open-field maze, DB-6 animals reduced center-zone entries by 42% in males and 20% in females (P < 0.05), indicating anxiety-like behavior. Gut microbiota profiling revealed significant increases in Lachnospiraceae sp. (+385%), Mucispirillum schaedleri (+174%), and Harryflintia sp. (+848%) and decreases in Muribaculaceae sp. (-36%), Bacteroides vulgatus (-69%), and Bilophila sp. (-81%; P < 0.50). Cecal SCFA concentrations declined in DB-6 animals (P = 0.01), including propionate (-18%) in males and isobutyrate (-37%) and isovalerate (-63%) in females. Significant correlations (P < 0.05) were found between these taxa and neurochemicals and behavioral changes.
conclusionsVB-6 deficiency alters gut microbiota composition, SCFA synthesis, and neurotransmitter balance, leading to anxiety-like behavior. These findings underscore the role of the gut microbiota in mediating VB-6-dependent gut-brain interactions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.