ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2025
Analytical comparison of 11 CE-IVD marked real-time PCR assays for Borrelia burgdorferi sensu lato detection.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Improving Molecular Detection of Tick-Borne Pathogens in Citizen-Collected Ticks.Pathogens (Basel, Switzerland) · 2026Article
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9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
purposeIn some precise clinical manifestations of Lyme borreliosis, PCR can be helpful for diagnosis: suspicion of atypical erythema migrans, borrelial lymphocytoma, acrodermatitis chronica atrophicans, Lyme arthritis or in very early seronegative neuroborreliosis. This study aimed to evaluate: (1) the analytical sensitivity of commercial real-time PCR kits for Borrelia burgdorferi sensu lato (Bbsl), (2) their specificity, and (3) their practicability.
methodsThe performances of 11 CE-IVD marked commercial kits and the French NRC Borrelia's in-house PCR were evaluated. PCRs were performed blindly on quantified DNA extracts, from 14 Bbsl strains corresponding to eight different genospecies, and on DNAs from relapsing fever (RF) Borrelia, Treponema, Leptospira, and 26 other micro-organisms.
resultsDNAs from Bbsl strains ranging from 10 to 10
conclusionThis study shows that the analytical performance and practicability of the kits evaluated are broadly in line, but that advanced molecular biology skills are required to interpret the results. Simultaneous detection of Lyme Borreliosis and RF agents without precise distinction may result in a risk of confusion in the choice of biological matrices and in the wording of the result returned to the clinician. Diagnostic laboratories must pay particular attention to the amplification confirmation strategy.
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