ArticleCurrent protocols2025
Comprehensive Tools for Culturing Blastocystis: A Standardized Resource for Research and Diagnostics.
Article in Current protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed.
- ExploringJournal of medical microbiology · 2026Review
- Commensal, pathogen, or passenger? Rethinking the role ofOne health (Amsterdam, Netherlands) · 2025Review
- Comprehensive Tools for Culturing Blastocystis: A Standardized Resource for Research and Diagnostics.Current protocols · 2025Article
- Microbial and metabolic signatures amongCurrent research in parasitology & vector-borne diseases · 2025Article
- The Microbiome Within a Microbe: Rethinking Blastocystis Biology.The Journal of eukaryotic microbiologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Blastocystis spp. is a widely prevalent anaerobic protozoan of uncertain pathogenicity found in the gastrointestinal tracts of over 1 billion people worldwide. Despite its potential significance in health and disease, Blastocystis spp. remains challenging to culture axenically due to its anaerobic nature and the diversity of its genetic subtypes. This manuscript presents a standardized toolkit for culturing Blastocystis spp. in xenic and axenic conditions, detailing protocols for the preparation of appropriate liquid and solid media, cryopreservation, and inoculation. By providing a comprehensive set of tools and methodologies, this work aims to streamline research on Blastocystis spp., enabling reproducibility, subtype characterization, and advancements in understanding its role in the gut microbiome and host health. © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Establishment of xenic Blastocystis culture from stool samples in liquid medium Basic Protocol 2: Subculturing from existing Blastocystis liquid culture Support Protocol 1: Preparation of modified Jones' medium for Blastocystis culturing Support Protocol 2: Preparation of trypticase-yeast extract-serum-gastric mucin-9 (TSGYM-9) medium for Blastocystis culturing Support Protocol 3: Preparation of liver extract-yeast extract-serum-gastric mucin (LYSGM) medium for Blastocystis culturing Basic Protocol 3: Subculturing xenic Blastocystis cultures in diphasic medium Support Protocol 4: Preparation of Robinson's medium for Blastocystis culturing Support Protocol 5: Preparation of Ringer's agar slants for Blastocystis culturing Basic Protocol 4: Axenization of Blastocystis cultures Basic Protocol 5: Subculturing axenic Blastocystis cultures in diphasic medium Support Protocol 6: Preparation of Boeck and Drbohlav's Locke-egg serum (LES) medium for Blastocystis culturing Support Protocol 7: Preparation of Boeck and Drbohlav's diphasic modified medium (BDMM) for Blastocystis culturing Basic Protocol 6: Establishment of axenic Blastocystis cultures in Iscove's modified Dulbecco's medium (IMDM) Basic Protocol 7: Establishment of axenic Blastocystis cultures in soft IMDM agar Basic Protocol 8: Establishment of axenic Blastocystis cultures on solid IMDM agar Basic Protocol 9: Optimized method for establishing axenic Blastocystis cultures on solid IMDM agar Basic Protocol 10: Establishment of axenic Blastocystis cultures in semi-solid Locke's agar Basic Protocol 11: Cryopreservation of xenic Blastocystis cultures Basic Protocol 12: Cryopreservation of axenic Blastocystis cultures Basic Protocol 13: Inoculation of liquid medium with xenic Blastocystis cultures from frozen stocks Basic Protocol 14: Inoculation of liquid medium with axenic Blastocystis cultures from frozen stocks.
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Registered trials
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