Evidence map›Paper›PMID 40810543›Full record

ArticleMicrobiology spectrum2025

Generating gnotobiotic bivalves: a new method on Manila clam (

Marialaura Gallo, Andrea Quagliariello, Giulia Dalla Rovere, Federica Maietti, Barbara Cardazzo, Luca Peruzza, Luca Bargelloni, Maria Elena Martino

Abstract read
In one paragraph

Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Marialaura GalloDepartment of Comparative Biomedicine and Food Science, University of Padova, Padua, Italy.
Andrea QuagliarielloDepartment of Comparative Biomedicine and Food Science, University of Padova, Padua, Italy.
Giulia Dalla RovereDepartment of Comparative Biomedicine and Food Science, University of Padova, Padua, Italy.
Federica MaiettiDepartment of Comparative Biomedicine and Food Science, University of Padova, Padua, Italy.
Barbara CardazzoDepartment of Comparative Biomedicine and Food Science, University of Padova, Padua, Italy.
Luca PeruzzaDepartment of Comparative Biomedicine and Food Science, University of Padova, Padua, Italy.
Luca BargelloniDepartment of Comparative Biomedicine and Food Science, University of Padova, Padua, Italy.ORCID 0000-0003-4351-2826
Maria Elena MartinoDepartment of Comparative Biomedicine and Food Science, University of Padova, Padua, Italy.ORCID 0000-0001-5038-5605

Funding

Fondazione Cassa di Risparmio di Padova e Rovigo (Fondazione Cariparo) C13C21000160005
6 · The paper itself

Abstract

Understanding how microbiomes influence the physiology of animal hosts requires detailed mechanistic insights, often obtained through gnotobiological approaches. Model organisms are central to this research, offering key advantages such as experimental tractability, reproducibility, and ease of manipulation. However, there is a lack of established gnotobiotic models for the marine environment-especially for bivalves, which play a critical role in ecosystem functioning. This gap is particularly important in the context of climate change, where harnessing microbiome resilience could mitigate environmental challenges and enhance host responses. In this study, we present a protocol to generate microbiome-depleted and gnotobiotic clams (

Indexed as

BacteriaBivalviaGerm-Free LifeMicrobiotaAnimalsRNA, Ribosomal, 16SRNA, Ribosomal, 16Sclamsgerm-free organismgnotobiologymicrobiomeRuditapes philippinarum

Identifiers

PMID40810543
PMCPMC12506633

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.