ArticleBiomedical optics express2025
Quantifying uncertainty in phasor-based time-domain fluorescence lifetime imaging microscopy.
Article in Biomedical optics express, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- FLIM quality metric visualization as a means to validate consistency across large-area non-homogeneous FLIM datasets.Methods and applications in fluorescence · 2026Article
- Chemical fluorophores for fluorescence lifetime imaging.Chemical Society reviews · 2026Review
- Multi-parameter fluorescence lifetime imaging for high-noise neuroscience applications.Biomedical optics express · 2026Article
- Quantifying uncertainty in phasor-based time-domain fluorescence lifetime imaging microscopy.Biomedical optics express · 2025Article
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Authors and funding
4 authors.
Funding
Abstract
The phasor approach to time-domain fluorescence lifetime imaging microscopy (FLIM) offers a powerful, fit-free method for analyzing complex fluorescence decay signals. However, its quantitative accuracy is fundamentally limited by noise-particularly photon shot noise-which introduces variability and deviations in lifetime estimation and fluorophore unmixing. In this study, we present a theoretical uncertainty model for phasor-based time-domain FLIM that analytically captures the propagation of shot noise and quantifies its impact on phasor coordinates and fluorophore weight estimation. We validate the model using Monte Carlo simulations and experimental data acquired from standard fluorescent dyes and biological tissue samples. Our model improves the overall reliability and efficiency of phasor-based time-domain FLIM, particularly in photon-limited imaging applications.
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Registered trials
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