ArticleFrontiers in genetics2025
Methanol fixation and tagmentation of RNA/DNA hybrids directly enable single-cell transcriptome sequencing.
Article in Frontiers in genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Objective: Single-cell transcriptome sequencing is a powerful tool for investigating cellular diversity in normal development and disease. However, prevalent methods predominantly employ 3'-end sequencing of transcripts, limiting the analysis of alternative splicing and other post-transcriptional processes. While full-length single-cell transcriptome sequencing methods, such as Smart-seq, offer more comprehensive information, but are restricted by low-throughput. To overcome these limitations, we propose a strategy that combines Methods: In this study, we utilized methanol fixation on cultured cells to evaluate RNA integrity and cellular preservation post-fixation. Results: Methanol fixation enables preservation of RNA and facilitates
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