Evidence map›Paper›PMID 40809201›Full record

ArticleJournal of thoracic disease2025

Optimizing core needle biopsy specimen collection techniques to enhance molecular testing adequacy in non-small cell lung cancer.

Zeyun Lin, Jiaxin Tang, Anzi Tan, Shiqi Tang, Yuqin Li, Yingying Gu, Chunping Liu, Mengshi Li, Zhucheng Chen, Yuying Chen and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of thoracic disease, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Zeyun Lin *Department of Pathology, Guangzhou Development District Hospital, Guangzhou, China.
Jiaxin Tang *State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Anzi TanState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Shiqi TangState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Yuqin LiDepartment of Clinical Laboratory, Dongguan Eighth People's Hospital, Dongguan, China.
Yingying GuState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Chunping LiuState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Mengshi LiState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Zhucheng ChenState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Yuying ChenState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Yuxin ZhangDepartment of Ultrasound, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Ping HeDepartment of Pathology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Qiuhua DengDepartment of Clinical Laboratory, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Wenhua LiangState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Jianxing HeState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Juhong JiangState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Core needle biopsy (CNB) is commonly used for histological diagnosis and molecular testing in patients with non-small cell lung cancer (NSCLC). However, the adequacy of CNB specimens is often compromised by low tumor cell content or insufficient nucleic acid quantity, which can negatively impact the success of downstream analyses. Moreover, variations in specimen collection techniques may further contribute to inconsistency in sample quality. This retrospective study aimed to assess and optimize CNB specimen collection methods to improve sample adequacy for molecular testing in NSCLC. Methods: A total of 546 CNB specimens from NSCLC patients were collected using three different techniques: (I) steam sterilization indicator cards; (II) polypropylene microporous membranes (PPMM) pieces for tissue adherence; and (III) direct rinsing of biopsy needles with sterilized buffer. Cell pellets were harvested from the residual fixative medium after CNB processing. Tumor cellularity was evaluated in formalin-fixed paraffin-embedded (FFPE) tissues and cell pellet samples from each method. Results: Adequacy rates for molecular testing were 86.4% in FFPE samples and 92.3% in cell pellet samples. Incorporating cell pellet analysis increased overall molecular testing adequacy to 95.2%. Among the evaluated techniques, the steam sterilization indicator cards cohort yielded the lowest adequacy rates for both FFPE and cell pellet samples. The PPMM technique achieved the highest adequacy for FFPE tissues, while the direct needle rinsing technique provided the highest DNA yields in cell pellet samples. Further, cell pellets demonstrated increased sensitivity for detecting actionable mutations compared to plasma-based liquid biopsies. Conclusions: The use of PPMMs significantly enhances CNB specimen quality and molecular testing adequacy in NSCLC. Routine collection of cell pellets from the residual fixative medium is recommended to maximize diagnostic effectiveness and improve clinical decision-making.

Indexed as

cell pelletcore needle biopsy (CNB)fixative mediummolecular testingNon-small cell lung cancer (NSCLC)

Identifiers

PMID40809201
PMCPMC12340258

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.