Evidence map›Paper›PMID 40808569›Full record

ArticleAnimal bioscience2026

Identification of genes and long non-coding RNAs for intramuscular and subcutaneous fat deposition in ducks by transcriptome analysis.

Tingting Zhou, Xunhao Meng, Wenshuang Liang, Min Xue, Tianqi Yang, Yong Jiang, Hao Bai, Guobin Chang, Guohong Chen, Zhixiu Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animal bioscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Tingting ZhouLaboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Xunhao MengLaboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Wenshuang LiangLaboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Min XueLaboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Tianqi YangLaboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Yong JiangLaboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Hao BaiLaboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Guobin ChangLaboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Guohong ChenLaboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Zhixiu WangLaboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.

Funding

China Agriculture Research System of MOF and MARA CARS-42-3National Key Research and Development Program of China 2023YFD1300301National Natural Science Foundation of China 32472890
6 · The paper itself

Abstract

objectiveFat deposition is an important factor that affects meat production and quality in livestock and poultry. Long non-coding RNAs (lncRNAs) play an important role in duck fat deposition. The purpose of this study was to identify key lncRNAs and mRNAs involved in fat deposition of meat ducks based on whole transcriptome sequencing for intramuscular preadipocyte (IMP-0), intramuscular adipocyte after 4 days of induction (IMP-4), subcutaneous preadipocyte (SCP-0), and subcutaneous adipocyte after 4 days of induction (SCP-4).

methodsDifferentially expressed mRNAs and lncRNAs were identified across groups through differential expression analysis, specific gene screening, and functional enrichment analysis. Subsequently, a lncRNA-mRNA co-expression network was constructed and key nodes were identified. Finally, preliminary expression validation was performed at the mRNA level.

resultsDifferential expression analysis revealed 1,419 mRNAs and 697 lncRNAs in the IMP-0-vs-IMP-4 comparison, and 2,307 mRNAs and 1,180 lncRNAs in the SCP-0-vs-SCP-4 comparison. Venn analysis identified unique differentially expressed genes for each group, including CHKA, PNPLA2, PLPP1, FABP4, ACSL5, UGT8, FAT1, and FADS2. Functional enrichment showed that the IMP-0-vs-IMP-4 group was significantly associated with regulation of the MAPK cascade, lipid binding, and arachidonic acid metabolism. The SCP-0-vs-SCP-4 group was notably enriched in beta-alanine metabolism, the Wnt signaling pathway, and lipid metabolic processes. Co-expression network analysis further constructed a network of 193 nodes and 275 edges for the IMP-0-vs-IMP-4 group, and a larger network of 564 nodes and 3,471 edges for the SCP-0-vs-SCP-4 group. Key lncRNAs, such as MSTRG.8652.4, MSTRG.15586.1, and MSTRG.6393.1, were identified based on their high connectivity degree.

conclusionTaken together, the current findings indicated that there are differentially regulated differential genes, lncRNAs, and enrichment pathways in IMP-0-vs-IMP-4 and SCP-0-vs-SCP-4. Because of being differentially regulated, some differential factors were significantly increased in expression in intramuscular adipocyte induction while significantly downregulated in subcutaneous adipocyte induction, such as FABP3, MSTRG.13937.5, and MSTRG.6393.1. Meanwhile, there were also some factors that were specifically regulated, CHKA, PLA2G4A, FADS2, MSTRG.13842.1, MSTRG.16051.2 and MSTRG.13842.1 were significantly downregulated only in subcutaneous adipocytes. This suggests that these lncRNAs and their target genes may play important roles in intramuscular fat and subcutaneous fat deposition.

Indexed as

Cell DifferentiationDuckIntramuscular PreadipocytesLong Non-coding RNASubcutaneous PreadipocytesWhole Transcriptome Sequencing

Identifiers

PMID40808569
PMCPMC12754461

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.