ArticleAnimal bioscience2026
Induction of germ cell-like cells from deleted in azoospermia-like enhanced green fluorescent protein gene knock-in chicken somatic cells via transgenic expression of pluripotency and germ cell-specific transcription factors.
Article in Animal bioscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
objectiveGerm cell identity is regulated by the coordinated action of multiple key transcription factors during embryonic development, which includes the induction and control of germ-line-specific gene expression. The expression of DEAD-box helicase 4 (DDX4) and deleted in azoospermia-like (DAZL) genes in chickens plays a pivotal role in germplasm formation and the specification of germ cell lineage from a totipotent genome. This study aimed to investigate the regulatory mechanisms underlying germ cell fate determination.
methodsLarge-scale gene expression profiling was conducted to screen and select critical transcription factors. This analysis identified differentially expressed genes in chicken primordial germ cells (PGCs), comprising 1,020 transcription factors. Additionally, we generated a chicken DF1 cell line featuring an enhanced green fluorescent protein (eGFP) reporter precisely knocked into the transcriptional start site of the DAZL gene using the CRISPR-Cas9 system, enabling real-time monitoring of DAZL expression during reprogramming.
resultsThrough analysis of transcription factor binding sites within approximately 10 kb upstream regions of DDX4 and DAZL, resulting in the selection of 10 candidate transcription factors for germ cell induction. Subsequently, the ten transcription factors identified as regulators of germ cell identity were transduced into the DAZL-knock-in eGFP DF1 cells. This approach led to the successful induction of eGFP-expressing cells in vitro, driven by the endogenous DAZL promoter. We conducted further characterization of these cells to confirm their germ cell-specific properties.
conclusionOur findings offer new insights into the transcriptional regulation of chicken germ cells by identifying key factors that activate DAZL expression. These results indicated valuable opportunities for advancing germ cell induction from somatic cells, with potential applications of in vitro models for studying germ cell-specific gene regulatory pathways in avian species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.