Evidence map›Paper›PMID 40807793›Full record

ArticleSensors (Basel, Switzerland)2025

Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions.

Mona Ghazalová, Pavlína Modlitbová, Ota Samek, Katarína Rebrošová, Martin Šiler, Jan Ježek, Zdeněk Pilát

Abstract read
In one paragraph

Article in Sensors (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mona GhazalováInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 00 Brno, Czech Republic.ORCID 0009-0006-7870-5862
Pavlína ModlitbováInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 00 Brno, Czech Republic.ORCID 0000-0003-4970-356X
Ota SamekInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 00 Brno, Czech Republic.ORCID 0000-0001-8188-7608
Katarína RebrošováDepartment of Microbiology, Faculty of Medicine of Masaryk University and St. Anne's, University Hospital, Pekařská 53, 656 91 Brno, Czech Republic.ORCID 0000-0002-6577-1864
Martin ŠilerInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 00 Brno, Czech Republic.ORCID 0000-0001-9040-4735
Jan JežekInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 00 Brno, Czech Republic.ORCID 0000-0002-5753-1676
Zdeněk PilátInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 00 Brno, Czech Republic.ORCID 0000-0001-9259-8393

Funding

EU Horizont Europe 101135175MEYS CZE LM2023050
6 · The paper itself

Abstract

This pilot study investigated the metabolic responses of five selected bacteria to physiological stress. Surface-enhanced Raman spectroscopy was used to analyze spectral changes associated with the release of adenine, a key metabolite indicative of stress conditions. Laboratory-synthesized spherical silver and gold nanoparticles, which remained stable over an extended period, were employed as enhanced surfaces. Bacterial cultures were analyzed under standard conditions and in the presence of a selected stressor-demineralized water-inducing osmotic stress. The results showed that the adenine signal originated from metabolites released into the surrounding environment rather than directly from the bacterial cell wall. The study confirms the suitability of these cost-effective and easily synthesized stable nanoparticles for the qualitative detection of bacterial metabolites using a commercially available Raman instrument.

Indexed as

AdenineBacteriaSpectrum Analysis, RamanStress, PhysiologicalGoldMetal NanoparticlesSilverAdenineGoldSilverAg-NPsAu-NPsEscherichia coliphenylalanineSERSStaphylococcus aureus

Identifiers

PMID40807793
PMCPMC12349020

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.