Evidence map›Paper›PMID 40807689›Full record

ArticleFoods (Basel, Switzerland)2025

Processed Pearl Millet Improves the Morphology and Gut Microbiota in Wistar Rats.

Jaqueline Maciel Vieira Theodoro, Lucimar Aguiar da Silva, Vinícius Parzanini Brilhante de São José, Nathaniel Baldwin Willis, Renata Celi Lopes Toledo, Mariana Grancieri, Carlos Wanderlei Piler Carvalho, Joseph Francis Pierre, Bárbara Pereira da Silva, Hércia Stampini Duarte Martino

Abstract read
In one paragraph

Article in Foods (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jaqueline Maciel Vieira TheodoroDepartment of Nutrition and Health, Federal University of Viçosa, Viçosa 36570900, Brazil.ORCID 0000-0001-8477-4705
Lucimar Aguiar da SilvaDepartment of Nutrition and Health, Federal University of Viçosa, Viçosa 36570900, Brazil.ORCID 0000-0002-3494-4988
Vinícius Parzanini Brilhante de São JoséDepartment of Nutrition and Health, Federal University of Viçosa, Viçosa 36570900, Brazil.ORCID 0000-0003-0298-0543
Nathaniel Baldwin WillisDepartment of Nutritional Sciences, College of Agriculture and Life Science, University of Wisconsin-Madison, Madison, WI 53706-1527, USA.ORCID 0000-0003-1401-8204
Renata Celi Lopes ToledoDepartment of Nutrition and Health, Federal University of Viçosa, Viçosa 36570900, Brazil.
Mariana GrancieriDepartment of Pharmacy and Nutrition, Federal University of Espírito Santo, Alegre 29500-000, Brazil.
Carlos Wanderlei Piler CarvalhoEmbrapa Food Technology, Rio de Janeiro 23020-470, Brazil.ORCID 0000-0002-7602-264X
Joseph Francis PierreDepartment of Nutritional Sciences, College of Agriculture and Life Science, University of Wisconsin-Madison, Madison, WI 53706-1527, USA.ORCID 0000-0002-4248-1290
Bárbara Pereira da SilvaDepartment of Nutrition and Health, Federal University of Viçosa, Viçosa 36570900, Brazil.ORCID 0000-0003-1096-456X
Hércia Stampini Duarte MartinoDepartment of Nutrition and Health, Federal University of Viçosa, Viçosa 36570900, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study evaluated the effect of pearl millet subjected to different processing on the intestinal health of rats. The animals were fed a standard iron-free diet (28 days) (hemoglobin depletion: 8.65 + 1.40 g/dL of hemoglobin). Subsequently, they were divided into four groups for hemoglobin repletion (21 days): standard diet + ferrous sulfate (SD + FS); standard diet + non-germinated open-pan cooked millet flour (SD + NGOPCMF); standard diet + germinated open-pan cooked millet flour (SD + GOPCMF); and standard diet + extrusion-cooked millet flour (SD + ECMF). Hemoglobin level did not differ among groups. The SD + NGOPCMF, SD + GOPCMF and SD + ECMF groups demonstrated a higher Chao index in the microbiome and a higher number and area of goblet cells and longitudinal muscle layer width. The SD + NGOPCMF and SD + GOPCMF groups demonstrated increased cecum weight, crypt depth, crypt thickness, and circular muscle layer width; lower fecal pH; and a higher relative abundance of Bacteroidota, while the SD + FS group showed the highest abundance of Actinobacteriota. The SD + GOPCMF group stood out for showing the lowest fecal pH, better α-diversity (Chao and Shannon index), and the highest width of the longitudinal muscle layer. In conclusion, pearl millet subjected to different processing, mainly germination, has the potential to improve the composition of the intestinal microbiota and the intestinal morphology in rats induced to iron deficiency.

Indexed as

extrusion cookinggerminationhemoglobin depletion/repletionintestinal health

Identifiers

PMID40807689
PMCPMC12346449

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.