Establishment of Singleplex and Duplex TaqMan RT-qPCR Detection Systems for Strawberry Mottle Virus (SMoV) and Strawberry Vein Banding Virus (SVBV).

Tengfei Xu, Dehang Gao, Mengmeng Wu, Hongqing Wang, Chengyong He

Abstract read

In one paragraph

Article in Plants (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Tengfei XuInstitute of Horticulture, Sichuan Academy of Agricultural Sciences, Chengdu 610000, China.ORCID 0000-0003-2310-4067

Dehang GaoDepartment of Fruit Science, College of Horticulture, China Agricultural University, Beijing 100107, China.ORCID 0009-0001-7249-4455

Mengmeng WuDepartment of Fruit Science, College of Horticulture, China Agricultural University, Beijing 100107, China.

Hongqing WangDepartment of Fruit Science, College of Horticulture, China Agricultural University, Beijing 100107, China.

Chengyong HeInstitute of Horticulture, Sichuan Academy of Agricultural Sciences, Chengdu 610000, China.ORCID 0000-0002-3963-8736

Funding

Sichuan Academy of Agricultural Sciences 2025ZYTS03Sichuan Academy of Agricultural Sciences NKYRCZX2025027

6 · The paper itself

Abstract

SMoV and SVBV are two major viruses that pose significant threats to the global strawberry industry. Both are latent viruses, making early detection difficult due to their uneven distribution and low concentration in host tissues. Traditional RT-PCR techniques are insufficient for precise and quantitative detection. In this study, TaqMan RT-qPCR detection systems for SMoV and SVBV were established for application in practical production settings, enabling accurate, rapid, and efficient detection of strawberry viruses. When viral accumulation in plants is low, the highly sensitive TaqMan RT-qPCR technique allows for accurate quantification, facilitating the early identification of infected plants and preventing large-scale outbreaks in cultivation areas. The development of a duplex TaqMan RT-qPCR assay enables simultaneous quantification of SMoV and SVBV in a single reaction, improving detection efficiency and providing technical support for risk assessment and effective control of strawberry viral diseases.

Indexed as

detection systemSMoVSVBVTaqMan RT-qPCR

Identifiers

PMID40805679

DOI10.3390/plants14152330

PMCPMC12349619

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.