ArticleScientific reports2025
RQdeltaCT: an open-source R package for relative quantification of gene expression using delta Ct methods.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- GeneQuantify: a web-based tool for qPCR gene expression and copy number variation analysis.Molecular and cellular biochemistry · 2026Article
- Cytokine-Family Biomarker Candidates for Small Abdominal Aortic Aneurysm Identified via Integrated mRNA and Protein Expression Profiling.International journal of molecular sciences · 2026Article
- Modulation of efflux pump gene expression in Enterococcus faecium by tungsten nanoparticles and multi-walled carbon nanotubes.Molecular biology reports · 2026Article
- Marine-Derived Padina Minor Extract Improves Lipid and Glucose Metabolism in Obese Rats: Evidence for PPARγ and ADIPOR1 Modulation.Nutrients · 2026Article
- Deleterious NKAP Mutations Are Associated with Musculoskeletal Abnormalities in Hemizygous Males and Skewed X Chromosome Inactivation in Heterozygous Females.International journal of molecular sciences · 2026Article
- Deciphering the Causative Role of a NovelBiomedicines · 2026Article
- Sub-inhibitory tobramycin concentration suppresses ToxA and LipA in single- and dual-species biofilms.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Evaluating the role of microplastics and wastewater in shaping Vibrio spp. and antibiotic resistance gene abundance in urban freshwaters.Scientific reports · 2025Article
- Molecular Signatures Related to Inflammation and Angiogenesis in Patients with Lower Extremity Artery Disease, Abdominal Aortic Aneurysm, and Varicose Veins: Shared and Distinct Pathways.International journal of molecular sciences · 2025Article
- Selection of stable reference genes for accurate reverse-transcription quantitative PCR in cotton-herbivore studies using virus-induced gene silencing.Scientific reports · 2025Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Relative quantification of gene expression is a fundamental method used in the molecular biology field to analyse real-time PCR data to determine transcriptional differences between groups of samples (or technical replicates of a single sample). The main methods used for relative quantification of gene expression are delta Ct methods that allow to compare means of gene expression between groups of samples without preparing a standard curve. Despite the availability of several tools for delta Ct methods, it is difficult to select the best one that would be characterized by sufficient flexibility and comprehensive functionality. Therefore, we developed the RQdeltaCT R package, which was designed to analyse real-time PCR data for the relative quantification of gene expression using delta Ct methods (including 2
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