Evidence map›Paper›PMID 40804190›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2025

Investigation of the canine distemper virus in dogs living at public kennels in Northern Brazil: validation of a detection assay.

Vitória Sahena Martins Souza Barbosa, Danielle Rodrigues de Deus, Renato da Silva Bandeira, Dielle Monteiro Teixeira, Marcelino Antônio Costa Maués, Márcia Janete de Fátima Mesquita de Figueiredo, Alexandre do Rosário Casseb, Luciana Damascena da Silva, Luana Silva Soares, Yvone Benchimol Gabbay and 2 more

Abstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Vitória Sahena Martins Souza BarbosaInstituto Evandro Chagas (IEC), Ministério da Saúde, Ananindeua, Brazil.ORCID http://orcid.org/0000-0002-0924-0284
Danielle Rodrigues de DeusInstituto Evandro Chagas (IEC), Ministério da Saúde, Ananindeua, Brazil.ORCID http://orcid.org/0000-0002-1153-5508
Renato da Silva BandeiraUniversidade Federal Do Oeste Do Pará (UFOPA), Ministério da Educação, Belém, Brazil.ORCID http://orcid.org/0000-0002-7988-3509
Dielle Monteiro TeixeiraInstituto Evandro Chagas (IEC), Ministério da Saúde, Ananindeua, Brazil.ORCID http://orcid.org/0000-0002-1007-9328
Marcelino Antônio Costa MauésCentro de Controle de Zoonoses (CCZ), Secretaria Municipal de Belém, Belém, Brazil.ORCID http://orcid.org/0000-0003-1532-4705
Márcia Janete de Fátima Mesquita de FigueiredoUniversidade Federal Rural da Amazônia (UFRA), Ministério da Educação, Belém, Brazil.ORCID http://orcid.org/0000-0003-3815-8439
Alexandre do Rosário CassebUniversidade Federal Rural da Amazônia (UFRA), Ministério da Educação, Belém, Brazil.ORCID http://orcid.org/0000-0001-5615-2423
Luciana Damascena da SilvaInstituto Evandro Chagas (IEC), Ministério da Saúde, Ananindeua, Brazil.ORCID http://orcid.org/0000-0002-2372-6520
Luana Silva SoaresInstituto Evandro Chagas (IEC), Ministério da Saúde, Ananindeua, Brazil.ORCID http://orcid.org/0000-0001-9509-4019
Yvone Benchimol GabbayInstituto Evandro Chagas (IEC), Ministério da Saúde, Ananindeua, Brazil.ORCID http://orcid.org/0000-0003-1242-0523
Jones Anderson Monteiro SiqueiraInstituto Evandro Chagas (IEC), Ministério da Saúde, Ananindeua, Brazil. jonessiqueira@iec.gov.br.ORCID http://orcid.org/0000-0002-6491-6590
Hugo Reis ResqueInstituto Evandro Chagas (IEC), Ministério da Saúde, Ananindeua, Brazil.ORCID http://orcid.org/0000-0001-6435-376X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Canine distemper (CD) is a severe infectious disease of dogs caused by the Canine Distemper Virus (CDV), a member of the genus Morbillivirus. This study aimed to investigate the circulation of CDV in dogs housed in two public kennels located in Belém in the Amazon region of Brazil. Fecal and rectal swab samples were collected between March 2019 and April, 2020. A one-step RT-PCR was initially standardized for CDV detection in fecal samples. Positive strains were partially sequenced using Sanger sequencing. A prevalence rate of 23.6% was observed, with 18.4% in healthy dogs and 46.7% in dogs with diarrhea. Phylogenetic analysis revealed that the samples were closely related to European strains and demonstrated the circulation of Brazilian strains belonging to the Europe 1/South America 1 lineage. This investigation successfully established a CDV diagnostic technique using RT-PCR in blood, feces, and urine samples. This study also verified the circulation of the virus in dogs from public kennels in an important city in the Brazilian Amazon.

Indexed as

DistemperDistemper Virus, CanineAnimalsBrazilDogsFecesPhylogenyReverse Transcriptase Polymerase Chain ReactionAmazonian ecosystemAsymptomatic conditionsCanis familiarisDiarrheaMorbilivirus

Identifiers

PMID40804190
PMCPMC12660541

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.