Evidence map›Paper›PMID 40803291›Full record

ArticleStem cell reports2025

Transcription factor-mediated germ cell induction in rats reveals ETV4 cooperates with germline specifiers.

Mami Oikawa, Hiroki Kojima, Hisato Kobayashi, Kenyu Iwatsuki, Hijiri Saito, Makoto Sanbo, Kazumi Nishioka, Tomoyuki Yamaguchi, Takuya Yamamoto, Kazuki Kurimoto and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Mami OikawaDivision of Mammalian Embryology, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan; Laboratory of Regenerative Medicine, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan.
Hiroki KojimaDivision of Mammalian Embryology, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan; Laboratory of Regenerative Medicine, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan; Division of Mammalian Embryogenesis, Department of Homeostatic Regulation, National Institute for Physiological Sciences, Okazaki, Aichi 444-8787, Japan.
Hisato KobayashiDepartment of Embryology, Nara Medical University, Kashihara, Nara 634-8521, Japan; Department of Medical Genome Science, Dokkyo Medical University, Mibu, Tochigi 321-0293, Japan.
Kenyu IwatsukiDivision of Mammalian Embryology, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan; Center for iPS Cell Research and Application, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan.
Hijiri SaitoDivision of Mammalian Embryology, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
Makoto SanboDivision of Mammalian Embryogenesis, Department of Homeostatic Regulation, National Institute for Physiological Sciences, Okazaki, Aichi 444-8787, Japan.
Kazumi NishiokaDivision of Mammalian Embryogenesis, Department of Homeostatic Regulation, National Institute for Physiological Sciences, Okazaki, Aichi 444-8787, Japan.
Tomoyuki YamaguchiLaboratory of Regenerative Medicine, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan.
Takuya YamamotoCenter for iPS Cell Research and Application, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan; Institute for the Advanced Study of Human Biology, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan; Medical-risk Avoidance based on iPS Cells Team, RIKEN Center for Advanced Intelligence Project, Sakyo-ku, Kyoto 606-8507, Japan.
Kazuki KurimotoDepartment of Embryology, Nara Medical University, Kashihara, Nara 634-8521, Japan.
Masumi HirabayashiDivision of Mammalian Embryogenesis, Department of Homeostatic Regulation, National Institute for Physiological Sciences, Okazaki, Aichi 444-8787, Japan.
Toshihiro KobayashiDivision of Mammalian Embryology, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan; Division of Mammalian Embryogenesis, Department of Homeostatic Regulation, National Institute for Physiological Sciences, Okazaki, Aichi 444-8787, Japan; The Graduate University of Advanced Studies, Okazaki, Aichi 444-8787, Japan. Electronic address: tkoba@nips.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The specification of primordial germ cells (PGCs) marks a crucial branchpoint in early embryonic development. Studying the molecular mechanisms governing this process is crucial for understanding reproduction and evolution. Here, we identify transcription factors essential for PGC specification in rats using an in vitro system to induce PGC-like cells (PGCLCs) from pluripotent cells. Overexpression of Tbxt, a key mesodermal factor activating the germ cell program in epiblast-like cells, induces functional rat PGCLCs, similar to mice. However, unlike in mice, overexpression of the PGC specifiers (Prdm14, Blimp1, and Ap2γ) alone is not sufficient in rats; additional Activin and WNT signals are necessary for PGCLC induction. Through a candidate screen, we identified the transcription factor Etv4 acting cooperatively with the three PGC specifiers. Our study provides insight into the mechanism behind germline segregation in mammals and underscores the importance of using the rat model in addition to mice.

Indexed as

Germ CellsProto-Oncogene Proteins c-etsTranscription FactorsAnimalsCell DifferentiationGene Expression Regulation, DevelopmentalMicePositive Regulatory Domain I-Binding Factor 1RatsPositive Regulatory Domain I-Binding Factor 1Proto-Oncogene Proteins c-etsTranscription Factorspluripotent stem cellprimordial germ cellrat

Identifiers

PMID40803291
PMCPMC12365847

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.