Evidence map›Paper›PMID 40802774›Full record

ArticleScience advances2025

Elusive tropical forest canopy diversity revealed through environmental DNA contained in rainwater.

Lucie Zinger, Anne-Sophie Benoiston, Yves Cuenot, Céline Leroy, Eliane Louisanna, Lucie Moreau, Frédéric Petitclerc, Finn Piatscheck, Jérôme Orivel, Cécile Richard-Hansen and 14 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Rainfall has contrasting effects on aquatic and terrestrial environmental DNA recovered from streams.Ecological applications : a publication of the Ecological Society of America · 2026
    Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Lucie ZingerCentre de Recherche sur la Biodiversité et l'Environnement (CRBE), UMR 5300 CNRS, IRD, INP, UT, Toulouse, France.ORCID 0000-0002-3400-5825
Anne-Sophie BenoistonCentre de Recherche sur la Biodiversité et l'Environnement (CRBE), UMR 5300 CNRS, IRD, INP, UT, Toulouse, France.ORCID 0000-0001-9446-5703
Yves CuenotCentre de Recherche sur la Biodiversité et l'Environnement (CRBE), UMR 5300 CNRS, IRD, INP, UT, Toulouse, France.ORCID 0000-0002-9755-811X
Céline LeroyCentre de Recherche sur la Biodiversité et l'Environnement (CRBE), UMR 5300 CNRS, IRD, INP, UT, Toulouse, France.ORCID 0000-0003-4859-8040
Eliane LouisannaUMR Ecologie des Forêts de Guyane (EcoFoG), INRAE, CNRS, AgroParisTech, CIRAD, Université de Guyane, Université des Antilles, Campus Agronomique, Kourou, France.ORCID 0000-0002-2082-5726
Lucie MoreauCentre de Recherche sur la Biodiversité et l'Environnement (CRBE), UMR 5300 CNRS, IRD, INP, UT, Toulouse, France.
Frédéric PetitclercUMR Ecologie des Forêts de Guyane (EcoFoG), INRAE, CNRS, AgroParisTech, CIRAD, Université de Guyane, Université des Antilles, Campus Agronomique, Kourou, France.ORCID 0000-0002-2015-1258
Finn PiatscheckUMR Ecologie des Forêts de Guyane (EcoFoG), INRAE, CNRS, AgroParisTech, CIRAD, Université de Guyane, Université des Antilles, Campus Agronomique, Kourou, France.ORCID 0000-0002-1796-6288
Jérôme OrivelCentre de Recherche sur la Biodiversité et l'Environnement (CRBE), UMR 5300 CNRS, IRD, INP, UT, Toulouse, France.ORCID 0000-0002-5636-3228
Cécile Richard-HansenUMR Ecologie des Forêts de Guyane (EcoFoG), INRAE, CNRS, AgroParisTech, CIRAD, Université de Guyane, Université des Antilles, Campus Agronomique, Kourou, France.ORCID 0000-0002-9353-791X
Lou Hansen-ChaffardOFB-Office Français de la Biodiversité/DRAS Campus agronomique de Kourou, Kourou, France.
Uxue SuescunCentre de Recherche sur la Biodiversité et l'Environnement (CRBE), UMR 5300 CNRS, IRD, INP, UT, Toulouse, France.
Valérie TroispouxUMR Ecologie des Forêts de Guyane (EcoFoG), INRAE, CNRS, AgroParisTech, CIRAD, Université de Guyane, Université des Antilles, Campus Agronomique, Kourou, France.ORCID 0000-0002-1118-1367
Frédéric BoyerLaboratoire d'Ecologie Alpine (LECA), UGA, UMR 5553 CNRS, University of Savoie Mont Blanc, Grenoble, France.ORCID 0000-0003-0021-9590
Jérôme ChaveCentre de Recherche sur la Biodiversité et l'Environnement (CRBE), UMR 5300 CNRS, IRD, INP, UT, Toulouse, France.ORCID 0000-0002-7766-1347
Thibaud DecaënsCEFE, Univ Montpellier, CNRS, EPHE, IRD, Montpellier, France.ORCID 0000-0003-0915-3882
Antoine FouquetCentre de Recherche sur la Biodiversité et l'Environnement (CRBE), UMR 5300 CNRS, IRD, INP, UT, Toulouse, France.ORCID 0000-0003-4060-0281
Johan PansuLaboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA), UMR 5023, Univ Lyon, Université Claude Bernard Lyon 1, CNRS, ENTPE, Villeurbanne, France.ORCID 0000-0003-0256-0258
Jérémy RaynaudCentre de Recherche sur la Biodiversité et l'Environnement (CRBE), UMR 5300 CNRS, IRD, INP, UT, Toulouse, France.ORCID 0009-0004-0606-0298
Rodolphe RougerieInstitut de Systématique, Évolution, Biodiversité (ISYEB), UMR 7205 Museum National d'Histoire Naturelle, CNRS, Sorbonne Université, EPHE-PSL, Université des Antilles, Paris, France.ORCID 0000-0003-0937-2815
Lucas SireInstitut de Systématique, Évolution, Biodiversité (ISYEB), UMR 7205 Museum National d'Histoire Naturelle, CNRS, Sorbonne Université, EPHE-PSL, Université des Antilles, Paris, France.ORCID 0000-0002-2911-6109
Heidy SchimannBiodiversité, Gènes et Communautés (BIOGECO), INRAE, Université de Bordeaux, Cestas, France.ORCID 0000-0002-9139-920X
Pierre TaberletLaboratoire d'Ecologie Alpine (LECA), UGA, UMR 5553 CNRS, University of Savoie Mont Blanc, Grenoble, France.ORCID 0000-0002-3554-5954
Amaia IribarCentre de Recherche sur la Biodiversité et l'Environnement (CRBE), UMR 5300 CNRS, IRD, INP, UT, Toulouse, France.ORCID 0000-0002-4709-648X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exploring the biodiversity hidden in tropical rainforests canopies represents a major frontier in biodiversity research yet remains challenging. Environmental DNA (eDNA) can revolutionize this field as it did already in various ecosystems. Here, we test the hypothesis that eDNA contained in canopy throughfall could be used to monitor this elusive diversity and detect anthropogenic disturbance. Using custom-made, low-cost rain collectors, we sampled rainwash eDNA in a mature Amazonian forest and a nearby tree plantation. We successfully detected eDNA from tropical woody and epiphyte plants, vertebrates (mammals, birds, and amphibians), and insects (e.g., mosquitoes, ants, and beetles). The taxonomic composition and diversity reflected disturbance, with significantly lower diversity in the plantation. Crucially, rainwash eDNA integrated biodiversity over a 10-day period in passive collectors and provided a local signature. This approach has thus potential for establishing a cost-effective monitoring system for tropical moist forest canopies, applicable in impact assessments and sustainable management.

Indexed as

BiodiversityDNA, EnvironmentalRainRainforestAnimalsEcosystemForestsTreesTropical ClimateDNA, Environmental

Identifiers

PMID40802774
PMCPMC12346338

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.