Evidence map›Paper›PMID 40802747›Full record

ArticleScience advances2025

Tracing the stepwise Darwinian evolution of a plant halogenase.

Colin Y Kim, David W Kastner, Andrew J Mitchell, Michael A Gutierrez, Jocelyn S Yao, Edwin N Neumann, Heather J Kulik, Jing-Ke Weng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Colin Y KimWhitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.ORCID 0000-0001-9583-9789
David W KastnerDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.ORCID 0000-0002-7766-4249
Andrew J MitchellWhitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.ORCID 0000-0003-3726-6569
Michael A GutierrezWhitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.ORCID 0000-0002-1089-5384
Jocelyn S YaoWhitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.ORCID 0000-0002-8007-8160
Edwin N NeumannWhitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.ORCID 0000-0003-4341-9816
Heather J KulikDepartment of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.ORCID 0000-0001-9342-0191
Jing-Ke WengWhitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.ORCID 0000-0003-3059-0075

Funding

Revealing Nature's Blueprints for Single-Site Catalysis of C-H Activation with First-principles Modeling and Machine LearningR35GM152027 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI Heather J. Kulik · 2024 to 2026
$1.2M
NIGMS NIH HHS R35 GM152027
6 · The paper itself

Abstract

Biohalogenation is rare in plant metabolism, with the Menispermaceae's chloroalkaloid acutumine being an exception. This involves a specialized dechloroacutumine halogenase (DAH) from the iron- and 2-oxoglutarate-dependent dioxygenase (2ODD) family. While DAH is presumed to have evolved from an ancestral 2ODD, how enzyme specialization arises through Darwinian processes remains a fundamental question in understanding metabolic evolution. Here, we investigate the evolutionary history of DAH using the chromosomal-level genome of

Indexed as

Evolution, MolecularPlant ProteinsPhylogenyPlant Proteins

Identifiers

PMID40802747
PMCPMC12346261

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.