ArticleArchives of endocrinology and metabolism2025
MiR-195-5p regulates oxidative stress and aerobic metabolism by directly downregulating GLS2 in high glucose-induced human lens epithelial cells.
Article in Archives of endocrinology and metabolism, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
objectiveTo investigated how miR-195-5p affects oxidative stress and modulates aerobic metabolism. MATERIALS AND
methodsMiR-195-5p plus GLS2 mRNA was identified by conducting real-time quantitative polymerase chain reaction. Western blotting was conducted to determine GLS2 protein expression. Corresponding kits were used to determine the concentrations of glutamate, reduced glutathione, oxidized glutathione, a-ketoglutarate, and adenosine triphosphate. The cell counting Kit-8 assay was performed to determine viability. Flow cytometry assay was performed to measure the reactive oxygen species content. Finally, a dual-luciferase reporter assay was conducted to confirm the interaction of miR-195-5p with GLS2 mRNA in the 3'UTR.
resultsIn high glucose-induced SRA01/04 cells, miR-195-5p was overexpressed, and GLS2 was downregulated. When miR-195-5p was upregulated, the levels of glutamate, reduced glutathione, a-ketoglutarate, and adenosine triphosphate, along with the reduced glutathione-to-oxidized glutathione ratio decreased, whereas the reactive oxygen species levels increased. Oxidative stress was ameliorated after miR-195-5p was downregulated. MiR-195-5p adversely controls the expression of GLS2 mRNA and protein. MiR-195-5p exacerbates oxidative damage and hinders aerobic metabolism by downregulating GLS2.
conclusionOxidative stress and aerobic metabolism in human lens epithelial cells were found to be regulated by miR-195-5p after the downregulation of GLS2.
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