ArticleLangmuir : the ACS journal of surfaces and colloids2025
Spatial Molecular Heterogeneity on Biofunctionalized Particles Quantified by Three-Dimensional Single-Molecule DNA-PAINT.
Article in Langmuir : the ACS journal of surfaces and colloids, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Single-molecule readout of reversible nanoswitches enables continuous monitoring of low biomarker concentrations.Nature communications · 2026Article
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Abstract
Quantifying and controlling the spatial molecular heterogeneity on biofunctionalized particles is essential for understanding and improving their functionality in bioscience applications. Here, we describe an analysis framework based on single-molecule localization microscopy that can quantitatively assess the spatial molecular properties of affinity molecules conjugated to particles. We performed 3D DNA-PAINT imaging on biofunctionalized particles and established analysis methods to correlate single-molecule data to the particle outer surfaces, count the number of conjugated molecules, and quantify the spatial distributions of the conjugated molecules. We show that imaging data combined with simulation-based molecular counting gives access to high densities of conjugated molecules and enables quantification of their spatial distributions. The analysis is exemplified for particles with a diameter of 1 μm functionalized with single-stranded DNA molecules via two bioconjugation methods, namely, streptavidin-biotin coupling and PLL-
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