Evidence map›Paper›PMID 40802304›Full record

ArticleJournal of applied oral science : revista FOB2025

Bee venom exhibits anti-cancer effects on tongue carcinoma cells by arresting cell cycle, inducing apoptosis, and suppressing cell migration.

Enas Sabry, Hagar M Zayed, Ola M Ezzatt, Iman Fathy, Hebatt-Allah S Elsayeh, Nashwa El-Khazragy, Suzan SeifAllah Ibrahim

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of applied oral science : revista FOB, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Enas SabryAin Shams University, Faculty of Dentistry, Department of Oral Medicine, Periodontology and Oral Diagnosis, Cairo, Egypt.ORCID http://orcid.org/0000-0002-3707-9600
Hagar M ZayedAin Shams University, Faculty of Dentistry, Department of Oral Medicine, Periodontology and Oral Diagnosis, Cairo, Egypt.ORCID http://orcid.org/0000-0002-4344-8249
Ola M EzzattAin Shams University, Faculty of Dentistry, Department of Oral Medicine, Periodontology and Oral Diagnosis, Cairo, Egypt.ORCID http://orcid.org/0000-0003-1656-5076
Iman FathyAin Shams University, Faculty of Dentistry, Department of Oral Biology, Cairo, Egypt.ORCID http://orcid.org/0000-0002-5219-2740
Hebatt-Allah S ElsayehBeekeeping Research Department, Plant Protection Research Institute, Agricultural Research Center, Ministry of Agriculture, Giza, Egypt.ORCID http://orcid.org/0009-0002-8772-9453
Nashwa El-KhazragyAin Shams University, Faculty of Medicine, Department of Clinical Pathology/Hematology, Cairo, Egypt.ORCID http://orcid.org/0000-0001-6646-4674
Suzan SeifAllah IbrahimAin Shams University, Faculty of Dentistry, Department of Oral Medicine, Periodontology and Oral Diagnosis, Cairo, Egypt.ORCID http://orcid.org/0000-0002-2335-6507

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTongue squamous cell carcinoma (TSCC) is an aggressive oral cancer with notable treatment resistance. This in vitro study investigated anti-cancer effects of honey bee venom (BV)-a mixture of bioactive compounds-on the human TSCC cell line. METHODOLOGY: The cytotoxicity of serial BV concentrations (0.01-100 µg/mL) was tested on the cultured human TSCC cell line (HNO-97) to determine the half-maximal inhibitory concentration (IC50) value. Group I (BV) included cells treated with IC50 of BV, and Group II (control) received no treatment; both were incubated for 48 hours. The apoptotic effect of BV was evaluated using the Annexin V assay and the BAX and BCL-2 gene expression. The BV effect on cell viability, proliferation, and division was evaluated by cell cycle assay. Additionally, Transwell migration assays were performed to demonstrate the potential impact of BV on cell migration.

resultsBV showed dose-dependent cytotoxicity and anti-proliferative activity on HNO-97 cells (IC50: 12.96 μg/mL). The treated group exhibited cell cycle arrest, reduced cell migration, significantly decreased BCL-2 gene expression (p=0.001), and increased BAX gene expression (p=0.03) compared to the untreated group.

conclusionBV demonstrated anti-cancer activity on human TSCC by inducing apoptosis and inhibiting cell migration. These findings warrant further preclinical investigations to evaluate BV as an alternative for current tongue carcinoma therapies.

Indexed as

Antineoplastic AgentsApoptosisBee VenomsCarcinoma, Squamous CellCell CycleCell MovementTongue Neoplasmsbcl-2-Associated X ProteinCell Cycle CheckpointsCell Line, TumorCell ProliferationCell SurvivalHumansInhibitory Concentration 50Proto-Oncogene Proteins c-bcl-2Reproducibility of ResultsAntineoplastic Agentsbcl-2-Associated X ProteinBee VenomsProto-Oncogene Proteins c-bcl-2

Identifiers

PMID40802304
PMCPMC12652438

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.